Direct and indirect effects of penguin feces on microbiomes in Antarctic ornithogenic soils

Yudong Guo, Nengfei Wang, Gaoyang Li, Gabriela Rosas, Jiaye Zang, Yue Ma, Jie Liu, Wenbing Han, Huansheng Cao

Research output: Contribution to journalArticle

Abstract

Expansion of penguin activity in maritime Antarctica, under ice thaw, increases the chances of penguin feces affecting soil microbiomes. The detail of such effects begins to be revealed. By comparing soil geochemistry and microbiome composition inside (one site) and outside (three sites) of the rookery, we found significant effects of penguin feces on both. First, penguin feces change soil geochemistry, causing increased moisture content (MC) of ornithogenic soils and nutrients C, N, P, and Si in the rookery compared to non-rookery sites, but not pH. Second, penguin feces directly affect microbiome composition in the rookery, not those outside. Specifically, we found 4,364 operational taxonomical units (OTUs) in 404 genera in six main phyla: Proteobacteria, Actinobacteria, Gemmatimonadetes, Acidobacteria, Chloroflexi, and Bacteroidetes. Although the diversity is similar among the four sites, the composition is different. For example, penguin rookery has a lower abundance of Acidobacteria, Chloroflexi, and Nitrospirae but a higher abundance of Bacteroidetes, Firmicutes, and Thermomicrobia. Strikingly, the family Clostridiaceae of Firmicutes of penguin-feces origin is most abundant in the rookery than non-rookery sites with two most abundant genera, Tissierella and Proteiniclasticum. Redundancy analysis showed all measured geochemical factors are significant in structuring microbiomes, with MC showing the highest correlation. We further extracted 21 subnetworks of microbes which contain 4,318 of the 4,364 OTUs using network analysis and are closely correlated with all geochemical factors except pH. Our finding f penguin feces, directly and indirectly, affects soil microbiome suggests an important role of penguins in soil geochemistry and microbiome structure of maritime Antarctica.

Original languageEnglish (US)
Article number552
JournalFrontiers in Microbiology
Volume9
Issue numberAPR
DOIs
StatePublished - Apr 3 2018

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Spheniscidae
Microbiota
Feces
Soil
Acidobacteria
Chloroflexi
Bacteroidetes
Proteobacteria
Actinobacteria
Ice

Keywords

  • Antarctica
  • Geochemical properties
  • Microbiome
  • Network
  • Ornithogenic soil
  • Penguin

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Direct and indirect effects of penguin feces on microbiomes in Antarctic ornithogenic soils. / Guo, Yudong; Wang, Nengfei; Li, Gaoyang; Rosas, Gabriela; Zang, Jiaye; Ma, Yue; Liu, Jie; Han, Wenbing; Cao, Huansheng.

In: Frontiers in Microbiology, Vol. 9, No. APR, 552, 03.04.2018.

Research output: Contribution to journalArticle

Guo, Yudong ; Wang, Nengfei ; Li, Gaoyang ; Rosas, Gabriela ; Zang, Jiaye ; Ma, Yue ; Liu, Jie ; Han, Wenbing ; Cao, Huansheng. / Direct and indirect effects of penguin feces on microbiomes in Antarctic ornithogenic soils. In: Frontiers in Microbiology. 2018 ; Vol. 9, No. APR.
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AU - Guo, Yudong

AU - Wang, Nengfei

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AU - Rosas, Gabriela

AU - Zang, Jiaye

AU - Ma, Yue

AU - Liu, Jie

AU - Han, Wenbing

AU - Cao, Huansheng

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