Differentiating the biomechanics of normal and diseased cells using isotropically etched silicon microstructures and atomic force microscopy

Mehdi Nikkhah, Jeannine S. Strobl, Masoud Agah

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper reports studying the differential biomechanical properties of HS68 normal human fibroblast cells and MDA-MB-231 human breast cancer cell (as the key cell types in tumour microenvironment [1]) using MEMS-based three dimensional (3-D) silicon microstructures and Atomic Force Microscopy (AFM). AFM results indicates that the cancer cells have significantly softer cytoskeletal structure (E=0.51±0.35 kPa) compared to normal fibroblast cells (E=1.86±1.13 kPa) supporting our previous observations and explaining the differential adhesion of normal and diseased cells to different compartments of the 3-D silicon microstructures.

Original languageEnglish (US)
Title of host publicationProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages1805-1807
Number of pages3
ISBN (Print)9780979806421
StatePublished - 2009
Externally publishedYes
Event13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 - Jeju, Korea, Republic of
Duration: Nov 1 2009Nov 5 2009

Other

Other13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009
CountryKorea, Republic of
CityJeju
Period11/1/0911/5/09

Fingerprint

Biomechanics
Silicon
Atomic force microscopy
Cells
Microstructure
Fibroblasts
MEMS
Tumors
Adhesion

Keywords

  • AFM
  • Breast cancer
  • Isotropic microchambers
  • MEMS
  • Silicon

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Nikkhah, M., Strobl, J. S., & Agah, M. (2009). Differentiating the biomechanics of normal and diseased cells using isotropically etched silicon microstructures and atomic force microscopy. In Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 1805-1807). Chemical and Biological Microsystems Society.

Differentiating the biomechanics of normal and diseased cells using isotropically etched silicon microstructures and atomic force microscopy. / Nikkhah, Mehdi; Strobl, Jeannine S.; Agah, Masoud.

Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2009. p. 1805-1807.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Nikkhah, M, Strobl, JS & Agah, M 2009, Differentiating the biomechanics of normal and diseased cells using isotropically etched silicon microstructures and atomic force microscopy. in Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, pp. 1805-1807, 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009, Jeju, Korea, Republic of, 11/1/09.
Nikkhah M, Strobl JS, Agah M. Differentiating the biomechanics of normal and diseased cells using isotropically etched silicon microstructures and atomic force microscopy. In Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society. 2009. p. 1805-1807
Nikkhah, Mehdi ; Strobl, Jeannine S. ; Agah, Masoud. / Differentiating the biomechanics of normal and diseased cells using isotropically etched silicon microstructures and atomic force microscopy. Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2009. pp. 1805-1807
@inproceedings{8a48222b01484d7f8aad11740fe84262,
title = "Differentiating the biomechanics of normal and diseased cells using isotropically etched silicon microstructures and atomic force microscopy",
abstract = "This paper reports studying the differential biomechanical properties of HS68 normal human fibroblast cells and MDA-MB-231 human breast cancer cell (as the key cell types in tumour microenvironment [1]) using MEMS-based three dimensional (3-D) silicon microstructures and Atomic Force Microscopy (AFM). AFM results indicates that the cancer cells have significantly softer cytoskeletal structure (E=0.51±0.35 kPa) compared to normal fibroblast cells (E=1.86±1.13 kPa) supporting our previous observations and explaining the differential adhesion of normal and diseased cells to different compartments of the 3-D silicon microstructures.",
keywords = "AFM, Breast cancer, Isotropic microchambers, MEMS, Silicon",
author = "Mehdi Nikkhah and Strobl, {Jeannine S.} and Masoud Agah",
year = "2009",
language = "English (US)",
isbn = "9780979806421",
pages = "1805--1807",
booktitle = "Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences",
publisher = "Chemical and Biological Microsystems Society",

}

TY - GEN

T1 - Differentiating the biomechanics of normal and diseased cells using isotropically etched silicon microstructures and atomic force microscopy

AU - Nikkhah, Mehdi

AU - Strobl, Jeannine S.

AU - Agah, Masoud

PY - 2009

Y1 - 2009

N2 - This paper reports studying the differential biomechanical properties of HS68 normal human fibroblast cells and MDA-MB-231 human breast cancer cell (as the key cell types in tumour microenvironment [1]) using MEMS-based three dimensional (3-D) silicon microstructures and Atomic Force Microscopy (AFM). AFM results indicates that the cancer cells have significantly softer cytoskeletal structure (E=0.51±0.35 kPa) compared to normal fibroblast cells (E=1.86±1.13 kPa) supporting our previous observations and explaining the differential adhesion of normal and diseased cells to different compartments of the 3-D silicon microstructures.

AB - This paper reports studying the differential biomechanical properties of HS68 normal human fibroblast cells and MDA-MB-231 human breast cancer cell (as the key cell types in tumour microenvironment [1]) using MEMS-based three dimensional (3-D) silicon microstructures and Atomic Force Microscopy (AFM). AFM results indicates that the cancer cells have significantly softer cytoskeletal structure (E=0.51±0.35 kPa) compared to normal fibroblast cells (E=1.86±1.13 kPa) supporting our previous observations and explaining the differential adhesion of normal and diseased cells to different compartments of the 3-D silicon microstructures.

KW - AFM

KW - Breast cancer

KW - Isotropic microchambers

KW - MEMS

KW - Silicon

UR - http://www.scopus.com/inward/record.url?scp=84901780752&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84901780752&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:84901780752

SN - 9780979806421

SP - 1805

EP - 1807

BT - Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences

PB - Chemical and Biological Microsystems Society

ER -