Differential sensitivity of mouse mononuclear phagocytes to CSF-1 and LPS: The potential in vivo relevance of enhanced IL-6 gene expression

Sonya J. Kamdar, Andrei Chapoval, Jessica Phelps, Jane A. Fuller, Robert Evans

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Abstract

In this report, we compared the responsiveness of subpopulations of mononuclear phagocytes (MNP) to the actions of the monocyte-macrophage colony-stimulating factor (CSF-1) and lipopolysaccharide (LPS), as measured by the expression of the IL-6 (Il6) gene. It was seen that neither monocytes nor elicited peritoneal macrophages (PMΦ) responded directly to CSF-1 compared with resident PMΦ that were induced to express high levels of Il6 mRNA and release IL-6 protein. Resident PMΦ released basal (constitutive) amounts of IL-6, while constitutive release by monocytes and elicited PMΦ was barely detectable. Monocytes and elicited PMΦ expressed similar levels of sensitivity to LPS, as measured by IL-6 release, and were less reactive than resident PM∅. When CSF-1 and LPS were added simultaneously to resident PMΦ, a dose-dependent synergistic release of IL-6 was seen. Elicited PMΦ also responded synergistically but required higher levels of CSF-1 and LPS, while monocytes failed to respond synergistically under any conditions. A similar synergistic effect was also seen in vivo when mice were injected with CSF-1 and LPS. Under these conditions, only resident peritoneal cells were shown to release IL-6 ex vivo while blood leukocytes and spleen cells released minimal amounts. These findings indicate that the stage of differentiation/maturation of MNP may be important for the ability of CSF-1 to render the cells sensitive to secondary stimulation, such as by LPS, and determines to what extent MNP subpopulations contribute to inflammatory responses in vivo.

Original languageEnglish (US)
Pages (from-to)165-172
Number of pages8
JournalCellular Immunology
Volume174
Issue number2
DOIs
StatePublished - Dec 15 1996
Externally publishedYes

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Macrophage Colony-Stimulating Factor
Peritoneal Macrophages
Phagocytes
Lipopolysaccharides
Interleukin-6
Gene Expression
Monocytes
Leukocytes
Spleen
Messenger RNA

ASJC Scopus subject areas

  • Immunology

Cite this

Differential sensitivity of mouse mononuclear phagocytes to CSF-1 and LPS : The potential in vivo relevance of enhanced IL-6 gene expression. / Kamdar, Sonya J.; Chapoval, Andrei; Phelps, Jessica; Fuller, Jane A.; Evans, Robert.

In: Cellular Immunology, Vol. 174, No. 2, 15.12.1996, p. 165-172.

Research output: Contribution to journalArticle

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