TY - JOUR
T1 - Differential localization and accumulation of the plant double stranded RNA-dependent protein kinase during virus infection
AU - Langland, Jeffrey
AU - Langland, Lisa
AU - Roth, Don
N1 - Funding Information:
Acknowledgements. This work was supported by a research grant from the National Science Foundation (MCB 9220617) to DAR.
PY - 1998/5
Y1 - 1998/5
N2 - Phosphorylation of the plant encoded, double-stranded (dsRNA)-dependent protein kinase (pPKR) is significantly enhanced over basal levels during early phases of virus and viroid infection. It is unclear, however, if corresponding pPKR protein levels are affected by pathogenesis. We now show that virus infection differentially effects pPKR protein levels. Significantly, cytosolic associated pPKR protein levels are induced 3-5 fold compared to levels in extracts from mock inoculated leaves. However, ribosome-associated pPKR protein levels from virus infected tissues decreased approximately 4-fold relative to levels in extracts from mock inoculated tissues. Further, the level of cytosolic-associated pPKR capable of binding to dsRNA declined during early TMV infection events suggesting that vital dsRNA's bind to pPKR in the cytosol during initial phases of pathogenesis.
AB - Phosphorylation of the plant encoded, double-stranded (dsRNA)-dependent protein kinase (pPKR) is significantly enhanced over basal levels during early phases of virus and viroid infection. It is unclear, however, if corresponding pPKR protein levels are affected by pathogenesis. We now show that virus infection differentially effects pPKR protein levels. Significantly, cytosolic associated pPKR protein levels are induced 3-5 fold compared to levels in extracts from mock inoculated leaves. However, ribosome-associated pPKR protein levels from virus infected tissues decreased approximately 4-fold relative to levels in extracts from mock inoculated tissues. Further, the level of cytosolic-associated pPKR capable of binding to dsRNA declined during early TMV infection events suggesting that vital dsRNA's bind to pPKR in the cytosol during initial phases of pathogenesis.
KW - DsRNA dependent protein kinase
KW - Protein phosphorylation
KW - TMV
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U2 - 10.1016/S0981-9428(98)80081-8
DO - 10.1016/S0981-9428(98)80081-8
M3 - Article
AN - SCOPUS:0032077382
SN - 0981-9428
VL - 36
SP - 395
EP - 400
JO - Plant Physiology and Biochemistry
JF - Plant Physiology and Biochemistry
IS - 5
ER -