Differential localization and accumulation of the plant double stranded RNA-dependent protein kinase during virus infection

Jeffrey Langland, Lisa Langland, Don Roth

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Phosphorylation of the plant encoded, double-stranded (dsRNA)-dependent protein kinase (pPKR) is significantly enhanced over basal levels during early phases of virus and viroid infection. It is unclear, however, if corresponding pPKR protein levels are affected by pathogenesis. We now show that virus infection differentially effects pPKR protein levels. Significantly, cytosolic associated pPKR protein levels are induced 3-5 fold compared to levels in extracts from mock inoculated leaves. However, ribosome-associated pPKR protein levels from virus infected tissues decreased approximately 4-fold relative to levels in extracts from mock inoculated tissues. Further, the level of cytosolic-associated pPKR capable of binding to dsRNA declined during early TMV infection events suggesting that vital dsRNA's bind to pPKR in the cytosol during initial phases of pathogenesis.

Original languageEnglish (US)
Pages (from-to)395-400
Number of pages6
JournalPlant Physiology and Biochemistry
Volume36
Issue number5
DOIs
StatePublished - Jan 1 1998
Externally publishedYes

Fingerprint

Plant RNA
eIF-2 Kinase
Double-Stranded RNA
double-stranded RNA
Virus Diseases
protein kinases
viruses
infection
Proteins
pathogenesis
proteins
Viruses and Viroids
Viroids
extracts
ribosomes
Ribosomes
cytosol
Cytosol
Protein Kinases
phosphorylation

Keywords

  • DsRNA dependent protein kinase
  • Protein phosphorylation
  • TMV

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

Cite this

Differential localization and accumulation of the plant double stranded RNA-dependent protein kinase during virus infection. / Langland, Jeffrey; Langland, Lisa; Roth, Don.

In: Plant Physiology and Biochemistry, Vol. 36, No. 5, 01.01.1998, p. 395-400.

Research output: Contribution to journalArticle

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