TY - JOUR
T1 - Differential evolution of substrates for an RNA enzyme in the presence and absence of its protein cofactor
AU - Liu, Fenyong
AU - Altman, Sidney
N1 - Funding Information:
Correspondence should be addressed to S. A. We thank our colleagues (in particular, Y. Li and Y. Yuan) for helpful discussions and for communicating results prior to publication, C. Guerrier-Takada for providing various plasmids, and S. Talbot for a gift of C5 protein. F. L. is a Parke-Davis fellow of the Life Sciences Research Foundation. Research in the laboratory of S. A. was supported by a grant (GM19422) from the United States Public Health Service.
PY - 1994/7/1
Y1 - 1994/7/1
N2 - Selection of substrates for an RNA enzyme, the catalytic subunit of RNAase P from E. coli, has been carried out by simulation of evolution in vitro in the presence and absence of the protein cofactor of the enzyme. In the presence of the protein, substrates resembling precursor tRNAs, which were readily cleaved by the catalytic RNA, were selected in addition to others, with different sequences and structures (one of which resembled the precursor to 4.5S RNA) that were not readily cleaved by the catalytic RNA alone. The ribonucleoprotein enzyme is more versatile than the RNA enzyme, and our results suggest that it and 4.5S RNA may have evolved after ancestral tRNAs.
AB - Selection of substrates for an RNA enzyme, the catalytic subunit of RNAase P from E. coli, has been carried out by simulation of evolution in vitro in the presence and absence of the protein cofactor of the enzyme. In the presence of the protein, substrates resembling precursor tRNAs, which were readily cleaved by the catalytic RNA, were selected in addition to others, with different sequences and structures (one of which resembled the precursor to 4.5S RNA) that were not readily cleaved by the catalytic RNA alone. The ribonucleoprotein enzyme is more versatile than the RNA enzyme, and our results suggest that it and 4.5S RNA may have evolved after ancestral tRNAs.
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U2 - 10.1016/0092-8674(94)90448-0
DO - 10.1016/0092-8674(94)90448-0
M3 - Article
C2 - 8020097
AN - SCOPUS:0028364761
SN - 0092-8674
VL - 77
SP - 1093
EP - 1100
JO - Cell
JF - Cell
IS - 7
ER -