Differential effects of mutations in the protein and RNA moieties of RNase P on the efficiency of suppression by various tRNA suppressors

Leif A. Kirsebom, Madeline F. Baer, Sidney Altman

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

We have studied the efficiency of suppression by tRNA suppressors in vivo in strains of Escherichia coli that harbor a mutation in the rnpA gene, the gene for the protein component (C5) of RNase P, and in strains that carry several different alleles of the rnpB gene, the gene for the RNA component (M1) of RNase P. Depending on the genetic background, different efficiencies of suppression by the various tRNA suppressors were observed. Thus, mutations in rnpA have separable and distinct effects from mutations in rnpB on the processing of tRNA precursors by RNase P. In addition, the efficiency of suppression by several derivatives of E. coli tRNATyr Su3 changed as the genetic background was altered.

Original languageEnglish (US)
Pages (from-to)879-888
Number of pages10
JournalJournal of Molecular Biology
Volume204
Issue number4
DOIs
StatePublished - Dec 20 1988
Externally publishedYes

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Ribonuclease P
Transfer RNA
Gene Components
RNA
Mutation
RNA, Transfer, Tyr
Escherichia coli
Proteins
RNA Precursors
Genes
Alleles
Genetic Background

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Differential effects of mutations in the protein and RNA moieties of RNase P on the efficiency of suppression by various tRNA suppressors. / Kirsebom, Leif A.; Baer, Madeline F.; Altman, Sidney.

In: Journal of Molecular Biology, Vol. 204, No. 4, 20.12.1988, p. 879-888.

Research output: Contribution to journalArticle

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