Different activation of immune-related genes in honey bee nurses and foragers (Apis mellifera)

Eva Marit Hystad Byhrø, Heli Salmela, Ana Vitlic, Ying Wang, Daniel Münch, Gro Amdam

Research output: Contribution to journalArticle

Abstract

Honey bee workers show very flexible aging patterns. Their typical behavioral progression, from nurse bees to forager bees, is associated with an onset of aging symptoms. Here, we studied how immune-activation can affect nurse and forager bees differently. Nurse and forager bees of identical chronological age were injected with vehicle control or the bacterial cell wall components lipopolysaccharide (LPS) or peptidoglycan (PGN). Next, we monitored survival and tested the expression of three immune-related genes. We confirm that forager bees die sooner than same-aged nurse bees, independent of the type of immune challenge. The relative gene expression patterns of two immune-related genes differed between the nurse and forager honey bees as well as between the treatment groups. Our findings support that nurse and forager honey bees can respond differently to immune challenge, despite a lack of detectable differences in mortality between treatments. This response was specifically associated with the workers’ behavioral caste, as the chronological age was similar among all tested animals.

Original languageEnglish (US)
JournalApidologie
DOIs
StatePublished - Jan 1 2019
Externally publishedYes

Fingerprint

worker honey bees
nurses
Apis mellifera
Apoidea
genes
honey bees
peptidoglycans
cell wall components
lipopolysaccharides
signs and symptoms (animals and humans)
gene expression
animals

Keywords

  • aging
  • defensin-2
  • honeybee
  • immune activation
  • vitellogenin

ASJC Scopus subject areas

  • Insect Science

Cite this

Different activation of immune-related genes in honey bee nurses and foragers (Apis mellifera). / Byhrø, Eva Marit Hystad; Salmela, Heli; Vitlic, Ana; Wang, Ying; Münch, Daniel; Amdam, Gro.

In: Apidologie, 01.01.2019.

Research output: Contribution to journalArticle

Byhrø, Eva Marit Hystad ; Salmela, Heli ; Vitlic, Ana ; Wang, Ying ; Münch, Daniel ; Amdam, Gro. / Different activation of immune-related genes in honey bee nurses and foragers (Apis mellifera). In: Apidologie. 2019.
@article{d228592d1ab543c085d28bc4e879ccc8,
title = "Different activation of immune-related genes in honey bee nurses and foragers (Apis mellifera)",
abstract = "Honey bee workers show very flexible aging patterns. Their typical behavioral progression, from nurse bees to forager bees, is associated with an onset of aging symptoms. Here, we studied how immune-activation can affect nurse and forager bees differently. Nurse and forager bees of identical chronological age were injected with vehicle control or the bacterial cell wall components lipopolysaccharide (LPS) or peptidoglycan (PGN). Next, we monitored survival and tested the expression of three immune-related genes. We confirm that forager bees die sooner than same-aged nurse bees, independent of the type of immune challenge. The relative gene expression patterns of two immune-related genes differed between the nurse and forager honey bees as well as between the treatment groups. Our findings support that nurse and forager honey bees can respond differently to immune challenge, despite a lack of detectable differences in mortality between treatments. This response was specifically associated with the workers’ behavioral caste, as the chronological age was similar among all tested animals.",
keywords = "aging, defensin-2, honeybee, immune activation, vitellogenin",
author = "Byhr{\o}, {Eva Marit Hystad} and Heli Salmela and Ana Vitlic and Ying Wang and Daniel M{\"u}nch and Gro Amdam",
year = "2019",
month = "1",
day = "1",
doi = "10.1007/s13592-019-00658-z",
language = "English (US)",
journal = "Apidologie",
issn = "0044-8435",
publisher = "Springer Science + Business Media",

}

TY - JOUR

T1 - Different activation of immune-related genes in honey bee nurses and foragers (Apis mellifera)

AU - Byhrø, Eva Marit Hystad

AU - Salmela, Heli

AU - Vitlic, Ana

AU - Wang, Ying

AU - Münch, Daniel

AU - Amdam, Gro

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Honey bee workers show very flexible aging patterns. Their typical behavioral progression, from nurse bees to forager bees, is associated with an onset of aging symptoms. Here, we studied how immune-activation can affect nurse and forager bees differently. Nurse and forager bees of identical chronological age were injected with vehicle control or the bacterial cell wall components lipopolysaccharide (LPS) or peptidoglycan (PGN). Next, we monitored survival and tested the expression of three immune-related genes. We confirm that forager bees die sooner than same-aged nurse bees, independent of the type of immune challenge. The relative gene expression patterns of two immune-related genes differed between the nurse and forager honey bees as well as between the treatment groups. Our findings support that nurse and forager honey bees can respond differently to immune challenge, despite a lack of detectable differences in mortality between treatments. This response was specifically associated with the workers’ behavioral caste, as the chronological age was similar among all tested animals.

AB - Honey bee workers show very flexible aging patterns. Their typical behavioral progression, from nurse bees to forager bees, is associated with an onset of aging symptoms. Here, we studied how immune-activation can affect nurse and forager bees differently. Nurse and forager bees of identical chronological age were injected with vehicle control or the bacterial cell wall components lipopolysaccharide (LPS) or peptidoglycan (PGN). Next, we monitored survival and tested the expression of three immune-related genes. We confirm that forager bees die sooner than same-aged nurse bees, independent of the type of immune challenge. The relative gene expression patterns of two immune-related genes differed between the nurse and forager honey bees as well as between the treatment groups. Our findings support that nurse and forager honey bees can respond differently to immune challenge, despite a lack of detectable differences in mortality between treatments. This response was specifically associated with the workers’ behavioral caste, as the chronological age was similar among all tested animals.

KW - aging

KW - defensin-2

KW - honeybee

KW - immune activation

KW - vitellogenin

UR - http://www.scopus.com/inward/record.url?scp=85068151592&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85068151592&partnerID=8YFLogxK

U2 - 10.1007/s13592-019-00658-z

DO - 10.1007/s13592-019-00658-z

M3 - Article

AN - SCOPUS:85068151592

JO - Apidologie

JF - Apidologie

SN - 0044-8435

ER -