Dietary fatty acids and cholesterol differentially modulate HDL cholesterol metabolism in Golden-Syrian hamsters

Suzanne E. Dorfman, Shu Wang, Sonia Vega-Lopez, Matti Jauhiainen, Alice H. Lichtenstein

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Dietary fatty acids alter HDL cholesterol concentrations presumably through mechanisms related to reverse cholesterol transport. The effect of dietary fats (coconut oil, butter, traditional stick margarine, soybean oil, canola oil) differing in fatty acid profile on this antiatherogenic process was assessed with respect to plasma lipids; exogenous and endogenous lecithin-cholesterol acyltransferase (LCAT), cholesterol ester transfer protein (CETP), phospholipid transfer protein (PLTP) activities; and LCAT, apolipoprotein (apo) A-I and scavenger receptor B class-1 (SR-B1) mRNA abundance. Golden-Syrian hamsters were fed a nonpurified (6.25 g/100 g fat) diet containing an additional 10 g/100 g experimental fat and 0.1 g/100 g cholesterol for 6 wk. Canola and soybean oils significantly lowered serum HDL cholesterol concentrations relative to butter. Canola oil, relative to butter, resulted in higher exogenous LCAT activity, and both soybean and canola oils significantly increased hepatic apo A-I and SR-B1 mRNA abundance. Butter, relative to margarine, coconut and soybean oils, significantly increased serum non-HDL cholesterol concentrations. Endogenous and exogenous LCAT, CETP, and PLTP activities did not differ in hamsters fed margarine or saturated fat diets, despite lower hepatic LCAT, apo A-I, and SR-B1 mRNA abundance, suggesting that changes in available substrate and/or modification to the LCAT protein may have been involved in lipoprotein changes. These results suggest that lower HDL cholesterol concentrations, as a result of canola and soybean oil feeding, may not be detrimental due to increases in components involved in the reverse cholesterol transport process in these hamsters and may retard the progression of atherosclerosis.

Original languageEnglish (US)
Pages (from-to)492-498
Number of pages7
JournalJournal of Nutrition
Volume135
Issue number3
StatePublished - Mar 2005
Externally publishedYes

Fingerprint

phosphatidylcholine-choline acyltransferase
Phosphatidylcholine-Sterol O-Acyltransferase
cholesterol metabolism
Mesocricetus
Soybean Oil
canola oil
high density lipoprotein cholesterol
dietary fat
HDL Cholesterol
Class B Scavenger Receptors
Butter
soybean oil
Fatty Acids
Cholesterol
butter
cholesterol
Margarine
apolipoprotein A-I
margarine
Apolipoprotein A-I

Keywords

  • Apolipoprotein A-I
  • Lecithin-cholesterol acyltransferase
  • Lipid transfer
  • Reverse cholesterol transport
  • Scavenger receptor B class 1

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Food Science

Cite this

Dietary fatty acids and cholesterol differentially modulate HDL cholesterol metabolism in Golden-Syrian hamsters. / Dorfman, Suzanne E.; Wang, Shu; Vega-Lopez, Sonia; Jauhiainen, Matti; Lichtenstein, Alice H.

In: Journal of Nutrition, Vol. 135, No. 3, 03.2005, p. 492-498.

Research output: Contribution to journalArticle

Dorfman, Suzanne E. ; Wang, Shu ; Vega-Lopez, Sonia ; Jauhiainen, Matti ; Lichtenstein, Alice H. / Dietary fatty acids and cholesterol differentially modulate HDL cholesterol metabolism in Golden-Syrian hamsters. In: Journal of Nutrition. 2005 ; Vol. 135, No. 3. pp. 492-498.
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