Development toward a triple-marker biosensor for diagnosing cardiovascular disease

Anna Deng, Daniel Matloff, Chi En Lin, David Probst, Theresa Broniak, Maryam Alsuwailem, Jeffrey LaBelle

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Cardiovascular disease (CVD) is the leading cause of death in the United States and is responsible for 30% of all deaths globally.1 The diagnosis and management of CVD requires monitoring of multiple biomarkers, which comprehensively represents the state of the disease. However, many assays for cardiac biomarkers today are complicated and laborious to perform. Rapid and sensitive biosensors capable of giving accurate measurements of vital cardiac bio-markers without complex procedures are thus in high demand. In the work presented below, rapid, label-free biosensor prototypes for three Food and Drug Administration–approved biomarkers are reported: B-type natriuretic peptide (BNP), cardiac troponin I (cTnI), and C-reactive protein (CRP). The sensors were prepared by immobilizing each biomarker’s antibody onto gold working electrodes with platinum counter and silver/silver chloride reference electrodes. The sensors were tested using electrochemical impedance spectroscopy (EIS), a femto-molar sensitive technique capable of label-free, multi-marker detection if a biomarker’s optimal frequency (OF) can be identified. The OFs of BNP, cTnI, and CRP were found to be 1.74, 37.56, and 253.9 Hz, respectively. The performance of the BNP biosensor was also evaluated in blood and achieved clinically relevant detection limits of 100 pg/mL.

Original languageEnglish (US)
Pages (from-to)169-178
Number of pages10
JournalCritical Reviews in Biomedical Engineering
Volume47
Issue number2
DOIs
StatePublished - Jan 1 2019

Keywords

  • Biomarkers
  • Cardiovascular disease
  • Electrochemical impedance spectroscopy
  • Imaginary impedance
  • Multi-marker biosensor
  • Optimal frequency

ASJC Scopus subject areas

  • Biomedical Engineering

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  • Cite this

    Deng, A., Matloff, D., Lin, C. E., Probst, D., Broniak, T., Alsuwailem, M., & LaBelle, J. (2019). Development toward a triple-marker biosensor for diagnosing cardiovascular disease. Critical Reviews in Biomedical Engineering, 47(2), 169-178. https://doi.org/10.1615/CritRevBiomedEng.2019026532