Development of protein chips based on self-assembled monolayer and protein A

Ling Sheng Jang; Hao Kai Keng; Yi Chu Hsu; Deirdre R. Meldrum

Development of protein chips based on self-assembled monolayer and protein A

Ling Sheng Jang, Hao Kai Keng, Yi Chu Hsu, Deirdre R. Meldrum

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

In order to improve the steric hindrances and less ordered structures in the long-chain SAMs, the SAM of 4,4-Dithiodibutyric acid (4,4- DTBA) was investigated to be a thin monolayer for the protein chip. This work presents the feasibility study of 4,4- DTBA as a monolayer of the protein chip based on gold surface. Characterizations of surface topography using atomic force microscopy (AFM), contact angle and fluorescence using protein A-FITC were performed to investigate the binding efficiency for the SAMs of 4,4- DTBA and 11- mercaptoundecanoic acid (11- MUA). The results show that the binding efficiency of 4,4- DTBA is higher than that of 11- MUA. The SAM of 4,4-DTBA can be used as a monolayer for the protein chip and may have a comparable binding efficiency.

Original language	English (US)
Title of host publication	ISCAS 2006
Subtitle of host publication	2006 IEEE International Symposium on Circuits and Systems, Proceedings
Pages	4353-4358
Number of pages	6
State	Published - 2006
Externally published	Yes
Event	ISCAS 2006: 2006 IEEE International Symposium on Circuits and Systems - Kos, Greece Duration: May 21 2006 → May 24 2006

Publication series

Name	Proceedings - IEEE International Symposium on Circuits and Systems
ISSN (Print)	0271-4310

Other

Other	ISCAS 2006: 2006 IEEE International Symposium on Circuits and Systems
Country/Territory	Greece
City	Kos
Period	5/21/06 → 5/24/06

ASJC Scopus subject areas

Electrical and Electronic Engineering

Cite this

Development of protein chips based on self-assembled monolayer and protein A. / Jang, Ling Sheng; Keng, Hao Kai; Hsu, Yi Chu et al.
ISCAS 2006: 2006 IEEE International Symposium on Circuits and Systems, Proceedings. 2006. p. 4353-4358 1693593 (Proceedings - IEEE International Symposium on Circuits and Systems).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Jang, LS, Keng, HK, Hsu, YC & Meldrum, DR 2006, Development of protein chips based on self-assembled monolayer and protein A. in ISCAS 2006: 2006 IEEE International Symposium on Circuits and Systems, Proceedings., 1693593, Proceedings - IEEE International Symposium on Circuits and Systems, pp. 4353-4358, ISCAS 2006: 2006 IEEE International Symposium on Circuits and Systems, Kos, Greece, 5/21/06.

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title = "Development of protein chips based on self-assembled monolayer and protein A",

abstract = "In order to improve the steric hindrances and less ordered structures in the long-chain SAMs, the SAM of 4,4-Dithiodibutyric acid (4,4- DTBA) was investigated to be a thin monolayer for the protein chip. This work presents the feasibility study of 4,4- DTBA as a monolayer of the protein chip based on gold surface. Characterizations of surface topography using atomic force microscopy (AFM), contact angle and fluorescence using protein A-FITC were performed to investigate the binding efficiency for the SAMs of 4,4- DTBA and 11- mercaptoundecanoic acid (11- MUA). The results show that the binding efficiency of 4,4- DTBA is higher than that of 11- MUA. The SAM of 4,4-DTBA can be used as a monolayer for the protein chip and may have a comparable binding efficiency.",

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series = "Proceedings - IEEE International Symposium on Circuits and Systems",

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T1 - Development of protein chips based on self-assembled monolayer and protein A

AU - Jang, Ling Sheng

AU - Keng, Hao Kai

AU - Hsu, Yi Chu

AU - Meldrum, Deirdre R.

PY - 2006

Y1 - 2006

N2 - In order to improve the steric hindrances and less ordered structures in the long-chain SAMs, the SAM of 4,4-Dithiodibutyric acid (4,4- DTBA) was investigated to be a thin monolayer for the protein chip. This work presents the feasibility study of 4,4- DTBA as a monolayer of the protein chip based on gold surface. Characterizations of surface topography using atomic force microscopy (AFM), contact angle and fluorescence using protein A-FITC were performed to investigate the binding efficiency for the SAMs of 4,4- DTBA and 11- mercaptoundecanoic acid (11- MUA). The results show that the binding efficiency of 4,4- DTBA is higher than that of 11- MUA. The SAM of 4,4-DTBA can be used as a monolayer for the protein chip and may have a comparable binding efficiency.

AB - In order to improve the steric hindrances and less ordered structures in the long-chain SAMs, the SAM of 4,4-Dithiodibutyric acid (4,4- DTBA) was investigated to be a thin monolayer for the protein chip. This work presents the feasibility study of 4,4- DTBA as a monolayer of the protein chip based on gold surface. Characterizations of surface topography using atomic force microscopy (AFM), contact angle and fluorescence using protein A-FITC were performed to investigate the binding efficiency for the SAMs of 4,4- DTBA and 11- mercaptoundecanoic acid (11- MUA). The results show that the binding efficiency of 4,4- DTBA is higher than that of 11- MUA. The SAM of 4,4-DTBA can be used as a monolayer for the protein chip and may have a comparable binding efficiency.

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BT - ISCAS 2006

T2 - ISCAS 2006: 2006 IEEE International Symposium on Circuits and Systems

Y2 - 21 May 2006 through 24 May 2006

ER -

Development of protein chips based on self-assembled monolayer and protein A

Abstract

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ASJC Scopus subject areas

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