Development of PCR assays targeting the genes involved in synthesis and assembly of the new Escherichia coli O174 and O177 O antigens

Lothar Beutin, Qingke Kong, Lu Feng, Quan Wang, Gladys Krause, Luciana Leomil, Qi Jin, Lei Wang

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

Escherichia coli O174 and O177 are newly described O serogroups which were reported as human pathogens. Identification of these strains by serotyping has been restricted, as the required sera are not commercially available. In this study, a collection of 13 E. coli O174 strains and 12 E. coli O177 strains was studied on the O:H serotypes and virulence markers. The O-antigen gene clusters of E. coli O174 and O177 were sequenced, and associated genes were assigned functions on the basis of homology. Two genes, each specific for E. coli O174 and O177, were identified. PCR assays based on the O-antigen-specific genes were developed and tested on 25 clinical and environmental isolates of those two serogroups as well as 26 isolates of other O serogroups. As little as 1 pg per μl of chromosomal DNA and as few as 0.1 CFU per g of pork and water samples were detected for either strain. The PCR assays established in this study were shown to be highly sensitive and reliable and could be the method of choice for detection of these two human pathogens from clinical, food, and other environmental samples.

Original languageEnglish (US)
Pages (from-to)5143-5149
Number of pages7
JournalJournal of Clinical Microbiology
Volume43
Issue number10
DOIs
StatePublished - Oct 2005

ASJC Scopus subject areas

  • Microbiology (medical)

Fingerprint Dive into the research topics of 'Development of PCR assays targeting the genes involved in synthesis and assembly of the new Escherichia coli O174 and O177 O antigens'. Together they form a unique fingerprint.

  • Cite this