Development of electrochemical methods to enzymatically detect traumatic brain injury biomarkers

Brittney A. Haselwood, Jeffrey LaBelle

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

As awareness of traumatic brain injury (TBI) increases, the need to detect mild forms and distinguish between the different severities of TBI becomes more apparent. The goal of this work is to develop a point-of-care sensor to detect whole blood biomarkers for rapid and sensitive diagnosis of TBI severity. Presented herein is the enzymatic detection of norepinephrine through the use of immobilization chemistry and impedance techniques. Sustained elevation of norepinephrine concentrations in the blood has been correlated to negative long-term outcomes in TBI cases, often resulting in permanent cognitive or physical deficits.Novel analysis techniques have been used to identify an optimal binding frequency (371.1Hz) of norepinephrine to the immobilized enzyme on a gold disk electrode. This form of analysis yielded a logarithmic fit characterized by exceptional responsivity (20.89ω/pgmL-1), reproducibility (R2=0.96), and lower limit of detection (98pg/mL) first in purified samples, then in rabbit whole blood solutions. Once the optimal binding frequency was determined, the preliminary use of an impedance-time technique was attempted in this work. This technique more closely resembles the amperometric detection method used in commercial self-monitoring blood glucose meters, allowing for continuous or instantaneous measurement of blood borne biomarkers without compromising sensitivity. Future directions include exploration of simultaneous multi-marker detection with the impedance-time technique and experimentation with novel mesoporous materials to filter large blood components.

Original languageEnglish (US)
Pages (from-to)752-756
Number of pages5
JournalBiosensors and Bioelectronics
Volume67
DOIs
StatePublished - May 5 2015

Fingerprint

Biomarkers
Brain
Blood
Norepinephrine
Electric Impedance
Point-of-Care Systems
Blood Glucose Self-Monitoring
Immobilized Enzymes
Mesoporous materials
Immobilization
Gold
Blood Glucose
Limit of Detection
Electrodes
Glucose
Traumatic Brain Injury
Rabbits
Enzymes
Monitoring
Sensors

Keywords

  • Electrochemistry
  • Enzyme
  • Impedance
  • Norepinephrine
  • Point-of-care technology
  • Traumatic brain injury

ASJC Scopus subject areas

  • Biophysics
  • Biomedical Engineering
  • Biotechnology
  • Electrochemistry

Cite this

Development of electrochemical methods to enzymatically detect traumatic brain injury biomarkers. / Haselwood, Brittney A.; LaBelle, Jeffrey.

In: Biosensors and Bioelectronics, Vol. 67, 05.05.2015, p. 752-756.

Research output: Contribution to journalArticle

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