Development of cellular absorptive tracers (CATs) for a quantitative characterization of microbial mass in flow systems

Jaeyoung Choi; Prasad Saripalli; Deirdre Meldrum; Ju Young Lee

doi:10.1016/j.biortech.2006.11.030

Development of cellular absorptive tracers (CATs) for a quantitative characterization of microbial mass in flow systems

Jaeyoung Choi, Prasad Saripalli, Deirdre Meldrum, Ju Young Lee

Research output: Contribution to journal › Article › peer-review

Abstract

A new method was developed for a simple non-destructive characterization of bacterial mass in flow systems. Results of partition and transport experiments showed that adsorption of a CAT molecule into the cellular mass resulted in its retardation during flow, which was a good measure of the biomass quantity and distribution. Three dyes, acridine orange (AO), toluidine blue (TB), and safranin O (SO), were chosen as CATs to demonstrate their utility to quantitatively characterize the biomass, its location and morphology in flow system. The results clearly demonstrated the applicability of AO, TB, and SO as cellular absorptive tracers in columnar flow experiments.

Original language	English (US)
Pages (from-to)	3630-3633
Number of pages	4
Journal	Bioresource Technology
Volume	98
Issue number	18
DOIs	https://doi.org/10.1016/j.biortech.2006.11.030
State	Published - Dec 1 2007
Externally published	Yes

Keywords

Biomass morphology
CXTFIT
Cellular absorptive tracers
Flow system

ASJC Scopus subject areas

Bioengineering
Environmental Engineering
Renewable Energy, Sustainability and the Environment
Waste Management and Disposal

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10.1016/j.biortech.2006.11.030

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abstract = "A new method was developed for a simple non-destructive characterization of bacterial mass in flow systems. Results of partition and transport experiments showed that adsorption of a CAT molecule into the cellular mass resulted in its retardation during flow, which was a good measure of the biomass quantity and distribution. Three dyes, acridine orange (AO), toluidine blue (TB), and safranin O (SO), were chosen as CATs to demonstrate their utility to quantitatively characterize the biomass, its location and morphology in flow system. The results clearly demonstrated the applicability of AO, TB, and SO as cellular absorptive tracers in columnar flow experiments.",

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AU - Choi, Jaeyoung

AU - Saripalli, Prasad

AU - Meldrum, Deirdre

AU - Lee, Ju Young

PY - 2007/12/1

Y1 - 2007/12/1

N2 - A new method was developed for a simple non-destructive characterization of bacterial mass in flow systems. Results of partition and transport experiments showed that adsorption of a CAT molecule into the cellular mass resulted in its retardation during flow, which was a good measure of the biomass quantity and distribution. Three dyes, acridine orange (AO), toluidine blue (TB), and safranin O (SO), were chosen as CATs to demonstrate their utility to quantitatively characterize the biomass, its location and morphology in flow system. The results clearly demonstrated the applicability of AO, TB, and SO as cellular absorptive tracers in columnar flow experiments.

AB - A new method was developed for a simple non-destructive characterization of bacterial mass in flow systems. Results of partition and transport experiments showed that adsorption of a CAT molecule into the cellular mass resulted in its retardation during flow, which was a good measure of the biomass quantity and distribution. Three dyes, acridine orange (AO), toluidine blue (TB), and safranin O (SO), were chosen as CATs to demonstrate their utility to quantitatively characterize the biomass, its location and morphology in flow system. The results clearly demonstrated the applicability of AO, TB, and SO as cellular absorptive tracers in columnar flow experiments.

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KW - Cellular absorptive tracers

KW - Flow system

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Development of cellular absorptive tracers (CATs) for a quantitative characterization of microbial mass in flow systems

Abstract

Keywords

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