Development of cellular absorptive tracers (CATs) for a quantitative characterization of microbial mass in flow systems

Jaeyoung Choi, Prasad Saripalli, Deirdre Meldrum, Ju Young Lee

Research output: Contribution to journalArticle


A new method was developed for a simple non-destructive characterization of bacterial mass in flow systems. Results of partition and transport experiments showed that adsorption of a CAT molecule into the cellular mass resulted in its retardation during flow, which was a good measure of the biomass quantity and distribution. Three dyes, acridine orange (AO), toluidine blue (TB), and safranin O (SO), were chosen as CATs to demonstrate their utility to quantitatively characterize the biomass, its location and morphology in flow system. The results clearly demonstrated the applicability of AO, TB, and SO as cellular absorptive tracers in columnar flow experiments.

Original languageEnglish (US)
Pages (from-to)3630-3633
Number of pages4
JournalBioresource Technology
Issue number18
StatePublished - Dec 1 2007
Externally publishedYes



  • Biomass morphology
  • Cellular absorptive tracers
  • Flow system

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

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