TY - JOUR
T1 - Development of B cells in scid mice with immunoglobulin transgenes
T2 - Implications for the Control of V(D)J recombination
AU - Chang, Yung
AU - Bosma, Gayle C.
AU - Bosma, Melvin J.
N1 - Funding Information:
We thank E. Cunningham, J. Dong, W. Schaut, and S. Shinton for technical assistance; T. Imanishi-Kari, D. Salter, and M. Weigert for wild-type transgenic mice; M. Cohn, D. Kappes, and M. Weigert for helpful discussion; M. Gellert, R. Hardy, D. Kappes. N. Rosenberg, D. Roth, and M. Weigert for critical review of the manuscript; and R. Diehl for typing the manuscript. This research was supported by grants from the National Institutes of Health (CA06927, A113323, and CA04946) and an appropriation from the Commonwealth of Pennsylvania
PY - 1995/6
Y1 - 1995/6
N2 - The inability of scid pro-B cells to progress to the pre-B and B cell stages is believed to be caused by a defective recombinase activity that fails to resolve chromosomal breaks resulting from attempted V(D)J recombination. In support of this model, we report that certain immunoglobulin transgenes, specifically those which strongly Inhibit endogenous VH-to-DJH and Vκ-to-Jκ rearrangement in wild-type mice, allow ccid pro-B cells to progress to the pre-B and B cell stages. This rescue of scid B cell differentiation is associated with a dramatic reduction in expression of the recombination activation genes, RAG1 and RAG2, and with reduced transcription of the K locus.
AB - The inability of scid pro-B cells to progress to the pre-B and B cell stages is believed to be caused by a defective recombinase activity that fails to resolve chromosomal breaks resulting from attempted V(D)J recombination. In support of this model, we report that certain immunoglobulin transgenes, specifically those which strongly Inhibit endogenous VH-to-DJH and Vκ-to-Jκ rearrangement in wild-type mice, allow ccid pro-B cells to progress to the pre-B and B cell stages. This rescue of scid B cell differentiation is associated with a dramatic reduction in expression of the recombination activation genes, RAG1 and RAG2, and with reduced transcription of the K locus.
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U2 - 10.1016/1074-7613(95)90005-5
DO - 10.1016/1074-7613(95)90005-5
M3 - Article
C2 - 7796294
AN - SCOPUS:0028979706
SN - 1074-7613
VL - 2
SP - 607
EP - 616
JO - Immunity
JF - Immunity
IS - 6
ER -