Development of a novel single sensor multiplexed marker assay

Jeffrey LaBelle, Ugur Korcan Demirok, Dharmendra R. Patel, Curtiss B. Cook

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

There is an increasing desire to measure multiple analytes simultaneously for disease management and detection. However, in the case of invasive devices, it would be better to obtain one small sample and immediately be able to detect the analytes rapidly, as in the case of self-monitoring blood glucose, without the need for additional steps, arrays, or reagents. Electrochemical impedance spectroscopy is used to measure the interaction between ultralow levels of analyte and molecular recognition element in a label-free and rapid manner. Gold nanoparticles were attached to antibodies against interleukin-12 and tumor necrosis factor-α, typical inflammatory markers found with near overlapping responses, on an impedance spectroscopy based biosensor. Cross-reactivity and specificity of tuned antibodies were verified using ELISA. Impedance frequency was quantified by concentration gradients of marker against the device. The natural impedance frequency for interleukin-12 (5.00 Hz) was tuned to a lower frequency four Hertz away from one another for better signal processing. This was accomplished without significantly altering the lower limits of detection (<4 pg ml-1 and ∼60 pg ml-1 for interleukin-12 and tumor necrosis factor-α, respectively), no cross-reactivity and specificity as determined by ELISAs. With modeling the nanoscale effects and further development, a larger tuning will be possible for making a better multiplexed sensor. Although interleukin-12 and TNF-α equivalent circuit calculations were modeled here, a sensor with the potential to measure multiple markers at once might offer a solution on the sensor front for simplified management of conditions such as diabetes, where both glucose and hemoglobin A1c values could be obtained.

Original languageEnglish (US)
Pages (from-to)1496-1501
Number of pages6
JournalAnalyst
Volume136
Issue number7
DOIs
StatePublished - Apr 7 2011

Fingerprint

Interleukin-12
Dielectric Spectroscopy
Assays
assay
sensor
Electric Impedance
Antibodies
tumor
antibody
Glucose
Sensors
glucose
Enzyme-Linked Immunosorbent Assay
spectroscopy
Blood Glucose Self-Monitoring
Equipment and Supplies
Molecular recognition
Antibody Specificity
Tumor Necrosis Factor-alpha
diabetes

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy
  • Electrochemistry
  • Biochemistry
  • Environmental Chemistry

Cite this

LaBelle, J., Demirok, U. K., Patel, D. R., & Cook, C. B. (2011). Development of a novel single sensor multiplexed marker assay. Analyst, 136(7), 1496-1501. https://doi.org/10.1039/c0an00923g

Development of a novel single sensor multiplexed marker assay. / LaBelle, Jeffrey; Demirok, Ugur Korcan; Patel, Dharmendra R.; Cook, Curtiss B.

In: Analyst, Vol. 136, No. 7, 07.04.2011, p. 1496-1501.

Research output: Contribution to journalArticle

LaBelle, J, Demirok, UK, Patel, DR & Cook, CB 2011, 'Development of a novel single sensor multiplexed marker assay', Analyst, vol. 136, no. 7, pp. 1496-1501. https://doi.org/10.1039/c0an00923g
LaBelle J, Demirok UK, Patel DR, Cook CB. Development of a novel single sensor multiplexed marker assay. Analyst. 2011 Apr 7;136(7):1496-1501. https://doi.org/10.1039/c0an00923g
LaBelle, Jeffrey ; Demirok, Ugur Korcan ; Patel, Dharmendra R. ; Cook, Curtiss B. / Development of a novel single sensor multiplexed marker assay. In: Analyst. 2011 ; Vol. 136, No. 7. pp. 1496-1501.
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