Development of a nomenclature system for a canine STR multiplex reagent kit

Bradley K. Tom, Mikko T. Koskinen, Melody Dayton, Anna Maria Mattila, Eric Johnston, Dennis Fantin, Sue Denise, Theresa Spear, David Glenn Smith, Jessica Satkoski, Bruce Budowle, Sree Kanthaswamy

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Despite the popularity of dogs in US households, canine DNA evidence remains largely untapped in forensic investigations partially because of the absence of well-defined forensic short tandem repeats (STRs), lack of standardized and validated PCR protocols, STR reagent kits, and poorly developed nomenclature. A nomenclature system was established based on internationally recognized recommendations for human forensic STRs for a recently developed canine STR reagent kit. Representative alleles were sequenced from each of the 18 STRs and the sex-typing marker included in the kit. This study also reflects on the impact of point mutations, insertions, and deletions within and outside the STR core repeat structures. An understanding of the STRs' sequence and repeat structures will enable development of a robust and reliable allele nomenclature and improve the accuracy and precision of allele fragment sizing in canine forensic profiling. The expected allele sizes have been calculated, and their repeat stuctures defined based on sequence information.

Original languageEnglish (US)
Pages (from-to)597-604
Number of pages8
JournalJournal of Forensic Sciences
Volume55
Issue number3
DOIs
StatePublished - May 1 2010
Externally publishedYes

Keywords

  • Complex repeats
  • Compound repeats
  • Domestic dog
  • Forensic science
  • Microsatellites
  • Nonhuman forensic DNA
  • Simple repeats

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Genetics

Fingerprint Dive into the research topics of 'Development of a nomenclature system for a canine STR multiplex reagent kit'. Together they form a unique fingerprint.

  • Cite this

    Tom, B. K., Koskinen, M. T., Dayton, M., Mattila, A. M., Johnston, E., Fantin, D., Denise, S., Spear, T., Smith, D. G., Satkoski, J., Budowle, B., & Kanthaswamy, S. (2010). Development of a nomenclature system for a canine STR multiplex reagent kit. Journal of Forensic Sciences, 55(3), 597-604. https://doi.org/10.1111/j.1556-4029.2010.01361.x