Development and validation of a 2,000-gene microarray for the fathead minnow (Pimephales promelas)

Patrick Larkin, Daniel L. Villeneuve, Iris Knoebl, Ann L. Miracle, Barbara J. Carter, Li Liu, Nancy D. Denslow, Gerald T. Ankley

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

Gene microarrays provide the field of ecotoxicology new tools to identify mechanisms of action of chemicals and chemical mixtures. Herein we describe the development and application of a 2,000-gene oligonucleotide microarray for the fathead minnow Pimephales promelas, a species commonly used in ecological risk assessments in North America. The microarrays were developed from various cDNA and subtraction libraries that we constructed. Consistency and reproducibility of the microarrays were documented by examining multiple technical replicates. To test application of the fathead minnow microarrays, gene expression profiles of fish exposed to 17β-estradiol, a well-characterized estrogen receptor (ER) agonist, were examined. For these experiments, adult male fathead minnows were exposed for 24 h to waterborne 17β-estradiol (40 or 100 ng/L) in a flow-through system, and gene expression in liver samples was characterized. Seventy-one genes were identified as differentially regulated by estradiol exposure. Examination of the gene ontology designations of these genes revealed patterns consistent with estradiol's expected mechanisms of action and also provided novel insights as to molecular effects of the estrogen. Our studies indicate the feasibility and utility of microarrays as a basis for understanding biological responses to chemical exposure in a model ecotoxicology test species.

Original languageEnglish (US)
Pages (from-to)1497-1506
Number of pages10
JournalEnvironmental Toxicology and Chemistry
Volume26
Issue number7
DOIs
StatePublished - Jul 1 2007
Externally publishedYes

Keywords

  • Ecotoxicology
  • Estradiol
  • Fathead minnow
  • Gene expression
  • Microarray

ASJC Scopus subject areas

  • Environmental Chemistry
  • Health, Toxicology and Mutagenesis

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