Development and Expression of Subunit Vaccines Against Viruses in Plants

Adrian Esqueda, Qiang Chen

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Various systems exist for the robust production of recombinant proteins. However, only a few systems are optimal for human vaccine protein production. Plant-based transient protein expression systems offer an advantageous alternative to costly mammalian cell culture-based systems and can perform posttranslational modifications due to the presence of an endomembrane system that is largely similar to that of the animal cell. Technological advances in expression vectors for transient expression in the last two decades have produced new plant expression systems with the flexibility and speed that cannot be matched by those based on mammalian or insect cell culture. The rapid and high-level protein production capability of transient expression systems makes them the optimal system to quickly and versatilely develop and produce vaccines against viruses such as 2019-nCoV that have sudden and unpredictable outbreaks. Here, expression of antiviral subunit vaccines in Nicotiana benthamiana plants via transient expression is demonstrated.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages25-38
Number of pages14
DOIs
StatePublished - 2021

Publication series

NameMethods in Molecular Biology
Volume2225
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Agroinfiltration
  • Nicotiana benthamiana
  • Plant-produced vaccines
  • Transient expression
  • Vaccines

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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