Deterministic Ratchet for Sub-micrometer (Bio)particle Separation

Daihyun Kim, Jinghui Luo, Edgar A. Arriaga, Alexandra Ros

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Resolving the heterogeneity of particle populations by size is important when the particle size is a signature of abnormal biological properties leading to disease. Accessing size heterogeneity in the sub-micrometer regime is particularly important to resolve populations of subcellular species or diagnostically relevant bioparticles. Here, we demonstrate a ratchet migration mechanism capable of separating sub-micrometer sized species by size and apply it to biological particles. The phenomenon is based on a deterministic ratchet effect, is realized in a microfluidic device, and exhibits fast migration allowing separation in tens of seconds. We characterize this phenomenon extensively with the aid of a numerical model allowing one to predict the speed and resolution of this method. We further demonstrate the deterministic ratchet migration with two sub-micrometer sized beads as model system experimentally as well as size-heterogeneous mouse liver mitochondria and liposomes as model system for other organelles. We demonstrate excellent agreement between experimentally observed migration and the numerical model.

Original languageEnglish (US)
Pages (from-to)4370-4379
Number of pages10
JournalAnalytical Chemistry
Volume90
Issue number7
DOIs
StatePublished - Apr 3 2018

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Numerical models
Mitochondria
Microfluidics
Liposomes
Liver
Particle size

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Deterministic Ratchet for Sub-micrometer (Bio)particle Separation. / Kim, Daihyun; Luo, Jinghui; Arriaga, Edgar A.; Ros, Alexandra.

In: Analytical Chemistry, Vol. 90, No. 7, 03.04.2018, p. 4370-4379.

Research output: Contribution to journalArticle

Kim, Daihyun ; Luo, Jinghui ; Arriaga, Edgar A. ; Ros, Alexandra. / Deterministic Ratchet for Sub-micrometer (Bio)particle Separation. In: Analytical Chemistry. 2018 ; Vol. 90, No. 7. pp. 4370-4379.
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