Abstract
Previous approaches of determining the molar concentration of nanoparticles often relied on the calibration factors extracted from standard samples or required prior knowledge regarding the geometry, optical, or chemical properties. In the present work, we proposed an absolute quantification method that determined the molar concentration of nano-objects without any calibration factor or prior knowledge. It was realized by monitoring the dynamic adsorption processes of individual nanoparticles with a high-speed surface plasmon resonance microscopy. In this case, diffusing nano-objects stochastically collided onto an adsorption interface and stayed there ("hit-n-stay" scenario), resulting in a semi-infinite diffusion system. The dynamic processes were analyzed with a theoretical model consisting of Fick's laws of diffusion and random-walk assumption. The quantification of molar concentration was achieved on the basis of an analytical expression, which involved only physical constants and experimental parameters. By using spherical polystyrene nanoparticles as a model, the present approach provided a molar concentration with excellent accuracy.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2380-2385 |
| Number of pages | 6 |
| Journal | Analytical Chemistry |
| Volume | 88 |
| Issue number | 4 |
| DOIs | |
| State | Published - Feb 16 2016 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Analytical Chemistry
Cite this
Determining the Absolute Concentration of Nanoparticles without Calibration Factor by Visualizing the Dynamic Processes of Interfacial Adsorption. / Wo, Xiang; Li, Zhimin; Jiang, Yingyan; Li, Minghe; Su, Yu Wen; Wang, Wei; Tao, Nongjian.
In: Analytical Chemistry, Vol. 88, No. 4, 16.02.2016, p. 2380-2385.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Determining the Absolute Concentration of Nanoparticles without Calibration Factor by Visualizing the Dynamic Processes of Interfacial Adsorption
AU - Wo, Xiang
AU - Li, Zhimin
AU - Jiang, Yingyan
AU - Li, Minghe
AU - Su, Yu Wen
AU - Wang, Wei
AU - Tao, Nongjian
PY - 2016/2/16
Y1 - 2016/2/16
N2 - Previous approaches of determining the molar concentration of nanoparticles often relied on the calibration factors extracted from standard samples or required prior knowledge regarding the geometry, optical, or chemical properties. In the present work, we proposed an absolute quantification method that determined the molar concentration of nano-objects without any calibration factor or prior knowledge. It was realized by monitoring the dynamic adsorption processes of individual nanoparticles with a high-speed surface plasmon resonance microscopy. In this case, diffusing nano-objects stochastically collided onto an adsorption interface and stayed there ("hit-n-stay" scenario), resulting in a semi-infinite diffusion system. The dynamic processes were analyzed with a theoretical model consisting of Fick's laws of diffusion and random-walk assumption. The quantification of molar concentration was achieved on the basis of an analytical expression, which involved only physical constants and experimental parameters. By using spherical polystyrene nanoparticles as a model, the present approach provided a molar concentration with excellent accuracy.
AB - Previous approaches of determining the molar concentration of nanoparticles often relied on the calibration factors extracted from standard samples or required prior knowledge regarding the geometry, optical, or chemical properties. In the present work, we proposed an absolute quantification method that determined the molar concentration of nano-objects without any calibration factor or prior knowledge. It was realized by monitoring the dynamic adsorption processes of individual nanoparticles with a high-speed surface plasmon resonance microscopy. In this case, diffusing nano-objects stochastically collided onto an adsorption interface and stayed there ("hit-n-stay" scenario), resulting in a semi-infinite diffusion system. The dynamic processes were analyzed with a theoretical model consisting of Fick's laws of diffusion and random-walk assumption. The quantification of molar concentration was achieved on the basis of an analytical expression, which involved only physical constants and experimental parameters. By using spherical polystyrene nanoparticles as a model, the present approach provided a molar concentration with excellent accuracy.
UR - http://www.scopus.com/inward/record.url?scp=84958214550&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84958214550&partnerID=8YFLogxK
U2 - 10.1021/acs.analchem.5b04386
DO - 10.1021/acs.analchem.5b04386
M3 - Article
AN - SCOPUS:84958214550
VL - 88
SP - 2380
EP - 2385
JO - Analytical Chemistry
JF - Analytical Chemistry
SN - 0003-2700
IS - 4
ER -