TY - JOUR
T1 - Determination of minimum sample size and discriminatory expression patterns in microarray data
AU - Hwang, Daehee
AU - Schmitt, William A.
AU - Stephanopoulos, George
AU - Stephanopoulos, Gregory
N1 - Funding Information:
This work was supported by the Engineering Research Program of the Office of Basic Energy Science at the Dept. of Energy, Grant No. DE-FG02-94ER-14487 and DE-FG02-99ER-15015 and NIH Grant number 1-R01-DK58533-01. We also acknowledge support by the Merck Genome Research Institute (MGRI).
PY - 2002/9
Y1 - 2002/9
N2 - Motivation: Transcriptional profiling using microarrays can reveal important information about cellular and tissue expression phenotypes, but these measurements are costly and time consuming. Additionally, tissue sample availability poses further constraints on the number of arrays that can be analyzed in connection with a particular disease or state of interest. It is therefore important to provide a method for the determination of the minimum number of microarrays required to separate, with statistical reliability, distinct disease states or other physiological differences. Results: Power analysis was applied to estimate the minimum sample size required for two-class and multi-class discrimination. The power analysis algorithm calculates the appropriate sample size for discrimination of phenotypic subtypes in a reduced dimensional space obtained by Fisher discriminant analysis (FDA). This approach was tested by applying the algorithm to existing data sets for estimation of the minimum sample size required for drawing certain conclusions on multi-class distinction with statistical reliability. It was confirmed that when the minimum number of samples estimated from power analysis is used, group mean in the FDA discrimination space are statistically different.
AB - Motivation: Transcriptional profiling using microarrays can reveal important information about cellular and tissue expression phenotypes, but these measurements are costly and time consuming. Additionally, tissue sample availability poses further constraints on the number of arrays that can be analyzed in connection with a particular disease or state of interest. It is therefore important to provide a method for the determination of the minimum number of microarrays required to separate, with statistical reliability, distinct disease states or other physiological differences. Results: Power analysis was applied to estimate the minimum sample size required for two-class and multi-class discrimination. The power analysis algorithm calculates the appropriate sample size for discrimination of phenotypic subtypes in a reduced dimensional space obtained by Fisher discriminant analysis (FDA). This approach was tested by applying the algorithm to existing data sets for estimation of the minimum sample size required for drawing certain conclusions on multi-class distinction with statistical reliability. It was confirmed that when the minimum number of samples estimated from power analysis is used, group mean in the FDA discrimination space are statistically different.
UR - http://www.scopus.com/inward/record.url?scp=0036738906&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036738906&partnerID=8YFLogxK
U2 - 10.1093/bioinformatics/18.9.1184
DO - 10.1093/bioinformatics/18.9.1184
M3 - Article
C2 - 12217910
AN - SCOPUS:0036738906
SN - 1367-4803
VL - 18
SP - 1184
EP - 1193
JO - Bioinformatics
JF - Bioinformatics
IS - 9
ER -