Abstract
Background: Knowledge of major histocompatibility complex (MHC) composition and distribution in rhesus macaque colonies is critical for management strategies that maximize the utility of this model for biomedical research. Methods: Variation within the Mamu-A and Mamu-B (class I) and DRB, DQA/B, and DPA/B (class II) regions of 379 animals from the Caribbean Primate Research Center's (CPRC) specific pathogen free (SPF) colony was examined using massively parallel sequencing. Results: Analyses of the 7 MHC loci revealed a background of Indian origin with high levels of variation despite past genetic bottlenecks. All loci exhibited mutual linkage disequilibria while conforming to Hardy–Weinberg expectations suggesting the achievement of mutation-selection balance. Conclusion: The CPRC's SPF colony is a significant resource for research on AIDS and other infectious agents. Characterizing colony-wide MHC variability facilitates the breeding and selection of animals bearing desired haplotypes and increases the investigator's ability to understand the immune responses mounted by these animals.
Original language | English (US) |
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Pages (from-to) | 379-387 |
Number of pages | 9 |
Journal | Journal of Medical Primatology |
Volume | 47 |
Issue number | 6 |
DOIs | |
State | Published - Dec 2018 |
Keywords
- Mamu haplotypes
- colony genetic structure
- genetic management
- major histocompatibility complex genotyping
- next generation sequence
ASJC Scopus subject areas
- Animal Science and Zoology
- General Veterinary