Determination of major histocompatibility class I and class II genetic composition of the Caribbean Primate Center specific pathogen-free rhesus macaque (Macaca mulatta) colony based on massively parallel sequencing

Sreetharan Kanthaswamy, Robert F. Oldt, Jillian Ng, David Glenn Smith, Melween I. Martínez, Carlos A. Sariol

Research output: Contribution to journalArticle

Abstract

Background: Knowledge of major histocompatibility complex (MHC) composition and distribution in rhesus macaque colonies is critical for management strategies that maximize the utility of this model for biomedical research. Methods: Variation within the Mamu-A and Mamu-B (class I) and DRB, DQA/B, and DPA/B (class II) regions of 379 animals from the Caribbean Primate Research Center's (CPRC) specific pathogen free (SPF) colony was examined using massively parallel sequencing. Results: Analyses of the 7 MHC loci revealed a background of Indian origin with high levels of variation despite past genetic bottlenecks. All loci exhibited mutual linkage disequilibria while conforming to Hardy–Weinberg expectations suggesting the achievement of mutation-selection balance. Conclusion: The CPRC's SPF colony is a significant resource for research on AIDS and other infectious agents. Characterizing colony-wide MHC variability facilitates the breeding and selection of animals bearing desired haplotypes and increases the investigator's ability to understand the immune responses mounted by these animals.

Original languageEnglish (US)
Pages (from-to)379-387
Number of pages9
JournalJournal of Medical Primatology
Volume47
Issue number6
DOIs
StatePublished - Dec 2018

Keywords

  • Mamu haplotypes
  • colony genetic structure
  • genetic management
  • major histocompatibility complex genotyping
  • next generation sequence

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

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