Determination of β-2 microglobulin levels in plasma using a high-throughput mass spectrometric immunoassay system

E. E. Niederkofler, K. A. Tubbs, K. Gruber, D. Nedelkov, U. A. Kiernan, Peter Williams, R. W. Nelson

Research output: Contribution to journalArticle

79 Scopus citations

Abstract

A high-throughput mass spectrometric immunoassay (MSIA) system for the analysis of proteins directly from biological fluids is reported. A 96-well-format robotic workstation equipped with antibody-derivatized affinity pipet tips was used for the parallel extraction of specific proteins from samples and subsequent deposition onto 96-well arrayed matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) targets. Interferences from nonspecifically bound proteins were minimized through choice of appropriate affinity pipet tip derivatization chemistries. Sample preparation for MALDI-TOFMS was enhanced through the use of hydrophobic/hydrophilic contrasting targets, which also presented functionalities found to promote matrix/analyte crystal growth. Automated mass spectrometry was used in the unattended acquisition of data, resulting in an analysis rate of ∼100 samples/h (biological fluid→data). The quantitative MSIA of β2m levels present in human plasma samples is given as illustration.

Original languageEnglish (US)
Pages (from-to)3294-3299
Number of pages6
JournalAnalytical chemistry
Volume73
Issue number14
DOIs
StatePublished - Jul 15 2001

ASJC Scopus subject areas

  • Analytical Chemistry

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    Niederkofler, E. E., Tubbs, K. A., Gruber, K., Nedelkov, D., Kiernan, U. A., Williams, P., & Nelson, R. W. (2001). Determination of β-2 microglobulin levels in plasma using a high-throughput mass spectrometric immunoassay system. Analytical chemistry, 73(14), 3294-3299. https://doi.org/10.1021/ac010143j