Abstract
A high-throughput mass spectrometric immunoassay (MSIA) system for the analysis of proteins directly from biological fluids is reported. A 96-well-format robotic workstation equipped with antibody-derivatized affinity pipet tips was used for the parallel extraction of specific proteins from samples and subsequent deposition onto 96-well arrayed matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) targets. Interferences from nonspecifically bound proteins were minimized through choice of appropriate affinity pipet tip derivatization chemistries. Sample preparation for MALDI-TOFMS was enhanced through the use of hydrophobic/hydrophilic contrasting targets, which also presented functionalities found to promote matrix/analyte crystal growth. Automated mass spectrometry was used in the unattended acquisition of data, resulting in an analysis rate of ∼100 samples/h (biological fluid→data). The quantitative MSIA of β2m levels present in human plasma samples is given as illustration.
Original language | English (US) |
---|---|
Pages (from-to) | 3294-3299 |
Number of pages | 6 |
Journal | Analytical Chemistry |
Volume | 73 |
Issue number | 14 |
DOIs | |
State | Published - Jul 15 2001 |
ASJC Scopus subject areas
- Analytical Chemistry