Detection of triclocarban and two co-contaminating chlorocarbanilides in US aquatic environments using isotope dilution liquid chromatography tandem mass spectrometry

Amir Sapkota, Jochen Heidler, Rolf Halden

Research output: Contribution to journalArticle

100 Citations (Scopus)

Abstract

The antimicrobial compound triclocarban (TCC; 3,4,4′-trichlorocarbanilide; CAS{music sharp sign} 101-20-2) is a high-production-volume chemical, recently suggested to cause widespread contamination of US water resources. To test this hypothesis, we developed an isotope dilution liquid chromatography electrospray ionization tandem mass spectrometry method for ultratrace analysis of TCC (0.9 ng/L detection limit) and analyzed low-volume water samples (200 mL) along with primary sludge samples from across the United States. All river water samples (100%) collected downstream of wastewater treatment plants had detectable levels of TCC, as compared to 56% of those taken upstream. Concentrations of TCC (mean±standard deviation) downstream of sewage treatment plants (84±110 ng/L) were significantly higher (P < 0.05; Wilcoxon rank sum test) than those of samples taken upstream (12±15 ng/L). Compared to surface water, mean TCC concentrations found in dried, primary sludge obtained from municipal sewage treatment plants in five states were six orders of magnitude greater (19,300±7100 μg/kg). Several river samples contained a co-contaminant, identified based on its chromatographic retention time, molecular base ion, and MS/MS fragmentation behavior as 4,4′-dichlorocarbanilide (DCC; CAS{music sharp sign} 1219-99-4). In addition to TCC and DCC, municipal sludge contained a second co-contaminant, 3,3′,4,4′-tetrachlorocarbanilide (TetraCC; CAS{music sharp sign} 4300-43-0). Both newly detected compounds were present as impurities (0.2%w/w each) in technical grade TCC (99%). Application of the new method for chlorocarbanilide analysis yielded TCC occurrence data for 13 US states, confirmed the role of sewage treatment plants as environmental inputs of TCC, and identified DCC and TetraCC as previously unrecognized pollutants released into the environment alongside TCC.

Original languageEnglish (US)
Pages (from-to)21-29
Number of pages9
JournalEnvironmental Research
Volume103
Issue number1
DOIs
StatePublished - Jan 2007
Externally publishedYes

Fingerprint

Sewage treatment plants
music
Liquid chromatography
Sewage
Tandem Mass Spectrometry
sewage treatment
Liquid Chromatography
Isotopes
aquatic environment
Dilution
Mass spectrometry
liquid chromatography
dilution
mass spectrometry
sludge
isotope
Impurities
pollutant
Music
Rivers

Keywords

  • Antimicrobials
  • Disinfectant
  • Emerging contaminants
  • LC-MS/MS
  • Phenyl urea compounds

ASJC Scopus subject areas

  • Environmental Science(all)

Cite this

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title = "Detection of triclocarban and two co-contaminating chlorocarbanilides in US aquatic environments using isotope dilution liquid chromatography tandem mass spectrometry",
abstract = "The antimicrobial compound triclocarban (TCC; 3,4,4′-trichlorocarbanilide; CAS{music sharp sign} 101-20-2) is a high-production-volume chemical, recently suggested to cause widespread contamination of US water resources. To test this hypothesis, we developed an isotope dilution liquid chromatography electrospray ionization tandem mass spectrometry method for ultratrace analysis of TCC (0.9 ng/L detection limit) and analyzed low-volume water samples (200 mL) along with primary sludge samples from across the United States. All river water samples (100{\%}) collected downstream of wastewater treatment plants had detectable levels of TCC, as compared to 56{\%} of those taken upstream. Concentrations of TCC (mean±standard deviation) downstream of sewage treatment plants (84±110 ng/L) were significantly higher (P < 0.05; Wilcoxon rank sum test) than those of samples taken upstream (12±15 ng/L). Compared to surface water, mean TCC concentrations found in dried, primary sludge obtained from municipal sewage treatment plants in five states were six orders of magnitude greater (19,300±7100 μg/kg). Several river samples contained a co-contaminant, identified based on its chromatographic retention time, molecular base ion, and MS/MS fragmentation behavior as 4,4′-dichlorocarbanilide (DCC; CAS{music sharp sign} 1219-99-4). In addition to TCC and DCC, municipal sludge contained a second co-contaminant, 3,3′,4,4′-tetrachlorocarbanilide (TetraCC; CAS{music sharp sign} 4300-43-0). Both newly detected compounds were present as impurities (0.2{\%}w/w each) in technical grade TCC (99{\%}). Application of the new method for chlorocarbanilide analysis yielded TCC occurrence data for 13 US states, confirmed the role of sewage treatment plants as environmental inputs of TCC, and identified DCC and TetraCC as previously unrecognized pollutants released into the environment alongside TCC.",
keywords = "Antimicrobials, Disinfectant, Emerging contaminants, LC-MS/MS, Phenyl urea compounds",
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T1 - Detection of triclocarban and two co-contaminating chlorocarbanilides in US aquatic environments using isotope dilution liquid chromatography tandem mass spectrometry

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N2 - The antimicrobial compound triclocarban (TCC; 3,4,4′-trichlorocarbanilide; CAS{music sharp sign} 101-20-2) is a high-production-volume chemical, recently suggested to cause widespread contamination of US water resources. To test this hypothesis, we developed an isotope dilution liquid chromatography electrospray ionization tandem mass spectrometry method for ultratrace analysis of TCC (0.9 ng/L detection limit) and analyzed low-volume water samples (200 mL) along with primary sludge samples from across the United States. All river water samples (100%) collected downstream of wastewater treatment plants had detectable levels of TCC, as compared to 56% of those taken upstream. Concentrations of TCC (mean±standard deviation) downstream of sewage treatment plants (84±110 ng/L) were significantly higher (P < 0.05; Wilcoxon rank sum test) than those of samples taken upstream (12±15 ng/L). Compared to surface water, mean TCC concentrations found in dried, primary sludge obtained from municipal sewage treatment plants in five states were six orders of magnitude greater (19,300±7100 μg/kg). Several river samples contained a co-contaminant, identified based on its chromatographic retention time, molecular base ion, and MS/MS fragmentation behavior as 4,4′-dichlorocarbanilide (DCC; CAS{music sharp sign} 1219-99-4). In addition to TCC and DCC, municipal sludge contained a second co-contaminant, 3,3′,4,4′-tetrachlorocarbanilide (TetraCC; CAS{music sharp sign} 4300-43-0). Both newly detected compounds were present as impurities (0.2%w/w each) in technical grade TCC (99%). Application of the new method for chlorocarbanilide analysis yielded TCC occurrence data for 13 US states, confirmed the role of sewage treatment plants as environmental inputs of TCC, and identified DCC and TetraCC as previously unrecognized pollutants released into the environment alongside TCC.

AB - The antimicrobial compound triclocarban (TCC; 3,4,4′-trichlorocarbanilide; CAS{music sharp sign} 101-20-2) is a high-production-volume chemical, recently suggested to cause widespread contamination of US water resources. To test this hypothesis, we developed an isotope dilution liquid chromatography electrospray ionization tandem mass spectrometry method for ultratrace analysis of TCC (0.9 ng/L detection limit) and analyzed low-volume water samples (200 mL) along with primary sludge samples from across the United States. All river water samples (100%) collected downstream of wastewater treatment plants had detectable levels of TCC, as compared to 56% of those taken upstream. Concentrations of TCC (mean±standard deviation) downstream of sewage treatment plants (84±110 ng/L) were significantly higher (P < 0.05; Wilcoxon rank sum test) than those of samples taken upstream (12±15 ng/L). Compared to surface water, mean TCC concentrations found in dried, primary sludge obtained from municipal sewage treatment plants in five states were six orders of magnitude greater (19,300±7100 μg/kg). Several river samples contained a co-contaminant, identified based on its chromatographic retention time, molecular base ion, and MS/MS fragmentation behavior as 4,4′-dichlorocarbanilide (DCC; CAS{music sharp sign} 1219-99-4). In addition to TCC and DCC, municipal sludge contained a second co-contaminant, 3,3′,4,4′-tetrachlorocarbanilide (TetraCC; CAS{music sharp sign} 4300-43-0). Both newly detected compounds were present as impurities (0.2%w/w each) in technical grade TCC (99%). Application of the new method for chlorocarbanilide analysis yielded TCC occurrence data for 13 US states, confirmed the role of sewage treatment plants as environmental inputs of TCC, and identified DCC and TetraCC as previously unrecognized pollutants released into the environment alongside TCC.

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