Detection of transplant vasculopathy in a rat aortic allograft model by fluorescence spectroscopic optical analysis

Alexander Christov; Erbin Dai; Liying Liu; Leslie W. Miller; Piers Nash; Alshad Lalani; Grant McFadden; Patric N. Nation; John Tulip; Alexandra Lucas

doi:10.1002/(SICI)1096-9101(1999)24:5<346::AID-LSM5>3.0.CO;2-K

Detection of transplant vasculopathy in a rat aortic allograft model by fluorescence spectroscopic optical analysis

Alexander Christov, Erbin Dai, Liying Liu, Leslie W. Miller, Piers Nash, Alshad Lalani, Grant McFadden, Patric N. Nation, John Tulip, Alexandra Lucas

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Background and Objective: Transplant vasculopathy is a leading cause of late cardiac graft loss. We have examined laser-induced fluorescence (LIF) spectroscopy as an optical diagnostic tool for detection of intimal plaque development and inflammatory cellular invasion in a rat model of aortic allograft transplant. Study Design/Materials and Methods: Infrarenal aortic segments were transplanted from Lewis to Sprague Dawley rats. A range of vasculopathy development was produced by treatment with a viral anti- inflammatory protein. LIF spectra were recorded from the intima of aortic implants at 28 days. Fluorescence intensity was analyzed for correlation with vasculopathy development. Results: Significant differences in LIF intensity at 400-450 nm (P ≤ 0.05 by ANOVA) were detected. LIF emission was correlated with plaque growth (R² = 0.980), vessel narrowing (R² = 0.964), and cellular invasion (R² = 0.971) by regression analysis. Conclusion: LIF optical analysis provides a nontraumatic diagnostic approach for detection of atherosclerosis prior to cardiac transplant or during development of vasculopathy after transplant.

Original language	English (US)
Pages (from-to)	346-359
Number of pages	14
Journal	Lasers in Surgery and Medicine
Volume	24
Issue number	5
DOIs	https://doi.org/10.1002/(SICI)1096-9101(1999)24:5<346::AID-LSM5>3.0.CO;2-K
State	Published - 1999
Externally published	Yes

Keywords

Atherosclerosis
Inflammation
Laser
Rejection
Transplant

ASJC Scopus subject areas

Surgery
Dermatology

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10.1002/(SICI)1096-9101(1999)24:5<346::AID-LSM5>3.0.CO;2-K

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title = "Detection of transplant vasculopathy in a rat aortic allograft model by fluorescence spectroscopic optical analysis",

abstract = "Background and Objective: Transplant vasculopathy is a leading cause of late cardiac graft loss. We have examined laser-induced fluorescence (LIF) spectroscopy as an optical diagnostic tool for detection of intimal plaque development and inflammatory cellular invasion in a rat model of aortic allograft transplant. Study Design/Materials and Methods: Infrarenal aortic segments were transplanted from Lewis to Sprague Dawley rats. A range of vasculopathy development was produced by treatment with a viral anti- inflammatory protein. LIF spectra were recorded from the intima of aortic implants at 28 days. Fluorescence intensity was analyzed for correlation with vasculopathy development. Results: Significant differences in LIF intensity at 400-450 nm (P ≤ 0.05 by ANOVA) were detected. LIF emission was correlated with plaque growth (R2 = 0.980), vessel narrowing (R2 = 0.964), and cellular invasion (R2 = 0.971) by regression analysis. Conclusion: LIF optical analysis provides a nontraumatic diagnostic approach for detection of atherosclerosis prior to cardiac transplant or during development of vasculopathy after transplant.",

keywords = "Atherosclerosis, Inflammation, Laser, Rejection, Transplant",

author = "Alexander Christov and Erbin Dai and Liying Liu and Miller, {Leslie W.} and Piers Nash and Alshad Lalani and Grant McFadden and Nation, {Patric N.} and John Tulip and Alexandra Lucas",

year = "1999",

doi = "10.1002/(SICI)1096-9101(1999)24:5<346::AID-LSM5>3.0.CO;2-K",

language = "English (US)",

volume = "24",

pages = "346--359",

journal = "Lasers in Surgery and Medicine",

issn = "0196-8092",

publisher = "Wiley-Liss Inc.",

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}

TY - JOUR

T1 - Detection of transplant vasculopathy in a rat aortic allograft model by fluorescence spectroscopic optical analysis

AU - Christov, Alexander

AU - Dai, Erbin

AU - Liu, Liying

AU - Miller, Leslie W.

AU - Nash, Piers

AU - Lalani, Alshad

AU - McFadden, Grant

AU - Nation, Patric N.

AU - Tulip, John

AU - Lucas, Alexandra

PY - 1999

Y1 - 1999

N2 - Background and Objective: Transplant vasculopathy is a leading cause of late cardiac graft loss. We have examined laser-induced fluorescence (LIF) spectroscopy as an optical diagnostic tool for detection of intimal plaque development and inflammatory cellular invasion in a rat model of aortic allograft transplant. Study Design/Materials and Methods: Infrarenal aortic segments were transplanted from Lewis to Sprague Dawley rats. A range of vasculopathy development was produced by treatment with a viral anti- inflammatory protein. LIF spectra were recorded from the intima of aortic implants at 28 days. Fluorescence intensity was analyzed for correlation with vasculopathy development. Results: Significant differences in LIF intensity at 400-450 nm (P ≤ 0.05 by ANOVA) were detected. LIF emission was correlated with plaque growth (R2 = 0.980), vessel narrowing (R2 = 0.964), and cellular invasion (R2 = 0.971) by regression analysis. Conclusion: LIF optical analysis provides a nontraumatic diagnostic approach for detection of atherosclerosis prior to cardiac transplant or during development of vasculopathy after transplant.

AB - Background and Objective: Transplant vasculopathy is a leading cause of late cardiac graft loss. We have examined laser-induced fluorescence (LIF) spectroscopy as an optical diagnostic tool for detection of intimal plaque development and inflammatory cellular invasion in a rat model of aortic allograft transplant. Study Design/Materials and Methods: Infrarenal aortic segments were transplanted from Lewis to Sprague Dawley rats. A range of vasculopathy development was produced by treatment with a viral anti- inflammatory protein. LIF spectra were recorded from the intima of aortic implants at 28 days. Fluorescence intensity was analyzed for correlation with vasculopathy development. Results: Significant differences in LIF intensity at 400-450 nm (P ≤ 0.05 by ANOVA) were detected. LIF emission was correlated with plaque growth (R2 = 0.980), vessel narrowing (R2 = 0.964), and cellular invasion (R2 = 0.971) by regression analysis. Conclusion: LIF optical analysis provides a nontraumatic diagnostic approach for detection of atherosclerosis prior to cardiac transplant or during development of vasculopathy after transplant.

KW - Atherosclerosis

KW - Inflammation

KW - Laser

KW - Rejection

KW - Transplant

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Detection of transplant vasculopathy in a rat aortic allograft model by fluorescence spectroscopic optical analysis

Abstract

Keywords

ASJC Scopus subject areas

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