Detection of transplant vasculopathy in a rat aortic allograft model by fluorescence spectroscopic optical analysis

Alexander Christov, Erbin Dai, Liying Liu, Leslie W. Miller, Piers Nash, Alshad Lalani, Douglas McFadden, Patric N. Nation, John Tulip, Alexandra Lucas

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background and Objective: Transplant vasculopathy is a leading cause of late cardiac graft loss. We have examined laser-induced fluorescence (LIF) spectroscopy as an optical diagnostic tool for detection of intimal plaque development and inflammatory cellular invasion in a rat model of aortic allograft transplant. Study Design/Materials and Methods: Infrarenal aortic segments were transplanted from Lewis to Sprague Dawley rats. A range of vasculopathy development was produced by treatment with a viral anti- inflammatory protein. LIF spectra were recorded from the intima of aortic implants at 28 days. Fluorescence intensity was analyzed for correlation with vasculopathy development. Results: Significant differences in LIF intensity at 400-450 nm (P ≤ 0.05 by ANOVA) were detected. LIF emission was correlated with plaque growth (R2 = 0.980), vessel narrowing (R2 = 0.964), and cellular invasion (R2 = 0.971) by regression analysis. Conclusion: LIF optical analysis provides a nontraumatic diagnostic approach for detection of atherosclerosis prior to cardiac transplant or during development of vasculopathy after transplant.

Original languageEnglish (US)
Pages (from-to)346-359
Number of pages14
JournalLasers in Surgery and Medicine
Volume24
Issue number5
DOIs
StatePublished - Jul 17 1999
Externally publishedYes

Fingerprint

Allografts
Lasers
Fluorescence
Transplants
Tunica Intima
Fluorescence Spectrometry
Sprague Dawley Rats
Atherosclerosis
Analysis of Variance
Anti-Inflammatory Agents
Regression Analysis
Growth
Proteins

Keywords

  • Atherosclerosis
  • Inflammation
  • Laser
  • Rejection
  • Transplant

ASJC Scopus subject areas

  • Surgery
  • Dermatology

Cite this

Detection of transplant vasculopathy in a rat aortic allograft model by fluorescence spectroscopic optical analysis. / Christov, Alexander; Dai, Erbin; Liu, Liying; Miller, Leslie W.; Nash, Piers; Lalani, Alshad; McFadden, Douglas; Nation, Patric N.; Tulip, John; Lucas, Alexandra.

In: Lasers in Surgery and Medicine, Vol. 24, No. 5, 17.07.1999, p. 346-359.

Research output: Contribution to journalArticle

Christov, Alexander ; Dai, Erbin ; Liu, Liying ; Miller, Leslie W. ; Nash, Piers ; Lalani, Alshad ; McFadden, Douglas ; Nation, Patric N. ; Tulip, John ; Lucas, Alexandra. / Detection of transplant vasculopathy in a rat aortic allograft model by fluorescence spectroscopic optical analysis. In: Lasers in Surgery and Medicine. 1999 ; Vol. 24, No. 5. pp. 346-359.
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