Detection of transplant vasculopathy in a rat aortic allograft model by fluorescence spectroscopic optical analysis

Alexander Christov, Erbin Dai, Liying Liu, Leslie W. Miller, Piers Nash, Alshad Lalani, Grant McFadden, Patric N. Nation, John Tulip, Alexandra Lucas

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


Background and Objective: Transplant vasculopathy is a leading cause of late cardiac graft loss. We have examined laser-induced fluorescence (LIF) spectroscopy as an optical diagnostic tool for detection of intimal plaque development and inflammatory cellular invasion in a rat model of aortic allograft transplant. Study Design/Materials and Methods: Infrarenal aortic segments were transplanted from Lewis to Sprague Dawley rats. A range of vasculopathy development was produced by treatment with a viral anti- inflammatory protein. LIF spectra were recorded from the intima of aortic implants at 28 days. Fluorescence intensity was analyzed for correlation with vasculopathy development. Results: Significant differences in LIF intensity at 400-450 nm (P ≤ 0.05 by ANOVA) were detected. LIF emission was correlated with plaque growth (R2 = 0.980), vessel narrowing (R2 = 0.964), and cellular invasion (R2 = 0.971) by regression analysis. Conclusion: LIF optical analysis provides a nontraumatic diagnostic approach for detection of atherosclerosis prior to cardiac transplant or during development of vasculopathy after transplant.

Original languageEnglish (US)
Pages (from-to)346-359
Number of pages14
JournalLasers in Surgery and Medicine
Issue number5
StatePublished - Jul 17 1999
Externally publishedYes


  • Atherosclerosis
  • Inflammation
  • Laser
  • Rejection
  • Transplant

ASJC Scopus subject areas

  • Surgery
  • Dermatology


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