Detection of psoriasin/S100A7 in the sera of patients with psoriasis

K. S. Anderson, J. Wong, K. Polyak, D. Aronzon, C. Enerbäck

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

Background: Psoriasis is a disease of dysregulated inflammation and epithelial hyperproliferation in the skin, involving both the innate and adaptive immune system. Psoriatic keratinocytes express high levels of psoriasin (S100A7), a small calcium-binding protein. Objectives: To determine if patients with active psoriasis have elevated serum levels of psoriasin and psoriasin-specific autoantibodies. Methods: Blood was collected from 14 patients with psoriasis vulgaris at the start of narrowband ultraviolet (UV) B therapy and from 11 of these patients every 2 weeks during the course of the UVB treatment. Patient and control sera were tested for psoriasin antigen levels by sandwich enzyme-linked immunosorbent assay, and for psoriasin autoantibody titres using recombinant purified psoriasin and overlapping peptides. Results: We confirmed strong and specific expression of psoriasin in psoriatic epidermis by immunohistochemistry. Systemic psoriasin antigen levels tended to be lower in patients (mean 213 ng mL-1) than in controls (mean 331 ng mL -1, P = 0.308) and decreased with increasing disease severity. Psoriasin-specific autoantibodies were detected in a subset of patients with psoriasis and healthy normal donors (mean 0.347 vs. 0.255 units, P = 0.246). The epitopes recognized by the autoantibodies were mapped to an external loop domain of the molecule but did not show corresponding T-cell immunogenicity. Conclusions: Although psoriasin is overexpressed in psoriatic skin lesions, systemic levels of psoriasin tended to be lower with increasing disease severity, which may be due to the presence of psoriasin-specific autoantibodies. Neither psoriasin nor psoriasin-specific autoantibodies appear to be promising serum biomarkers for clinical psoriasis.

Original languageEnglish (US)
Pages (from-to)325-332
Number of pages8
JournalBritish Journal of Dermatology
Volume160
Issue number2
DOIs
StatePublished - Feb 1 2009
Externally publishedYes

Keywords

  • Autoantibodies
  • Biomarker
  • Psoriasis
  • S100A7

ASJC Scopus subject areas

  • Dermatology

Fingerprint Dive into the research topics of 'Detection of psoriasin/S100A7 in the sera of patients with psoriasis'. Together they form a unique fingerprint.

Cite this