Detection of differentially expressed genes in human colon carcinoma cells treated with a selective COX-2 inhibitor

Zhonghua Zhang, Raymond N. Dubois

Research output: Contribution to journalArticle

81 Scopus citations

Abstract

Numerous reports suggest that use of nonsteroidal anti-inflammatory drugs (NSAIDs) decrease mortality from colorectal cancer. To better understand all of the mechanisms underlying this effect, the global pattern of gene expression in colon carcinoma cells following treatment with NS-398, a selective cyclo-oxygenase-2 inhibitor was evaluated. We utilized suppression subtractive hybridization combined with differential screening to identify genes whose expression was affected following treatment. Among the subtracted cDNA fragments confirmed as differentially expressed, there were two which are known to be involved in the regulation of cell adhesion (human FAT and proto-cadherin-7). We identified two other genes whose levels were decreased and these are known to be involved in the regulation of cell proliferation (cyclin K and p-100). We identified additional genes which are involved in different signaling pathways which regulate programmed cell death (Dynamin 2, Pdcd4 and LIP.1). These results provide evidence that some of the effects of NS-398 on carcinoma cells may be due to modulation of genes which regulate programmed cell death, cell proliferation and cell-cell communication. Additional studies are underway to determine the biological function of the novel genes that were identified.

Original languageEnglish (US)
Pages (from-to)4450-4456
Number of pages7
JournalOncogene
Volume20
Issue number33
DOIs
StatePublished - Jul 27 2001

Keywords

  • Colon cancer
  • Cyclo-oxygenase-2
  • NSAID
  • Subtraction cloning

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

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