TY - JOUR
T1 - Detection of copper ions in drinking water using the competitive adsorption of proteins
AU - Wang, Ran
AU - Wang, Wei
AU - Ren, Hao
AU - Chae, Junseok
N1 - Funding Information:
SPR is a very sensitive instrument, which detects minute refractive index change at the vicinity of gold surface. This work utilizes SPR to detect Cu mg/L Cu mg/L, in National Primary Drinking Water Regulations published by United States Environment Protection Agency (EPA). The SPR biosensor is characterized for 5 different heavy metal ions, Cu mDeg suggesting the biosensor may be used to detect Cu 2+ , a common contaminant in tap water, using competitive adsorption of proteins, termed the Vroman effect. Cu 2+ -denatured albumin has lower affinity than native albumin to gold surface, which can be replaced by a sequence of adsorption/desorption phenomena. This sequence of adsorption/desorption is monitored by SPR in real time to quantify the concentration of Cu 2+ ions in water samples, including PBS, tap water, DI water, and bottled water. The affinity change of protein denaturation by Cu 2+ is demonstrated by Circular Dichroism and gold nanoparticle (AuNP) flocculation assay. The SPR biosensor can detect down to 0.1 2+ , lower than the Maximum Contaminant Level (MCL), 1.3 2+ , Fe 3+ , Mn 2+ , Pb 2+ , and Hg 2+ , for its selectivity. At MCL, the SPR biosensor produces 13.5±0.4, 1.5±0.4, 0, 0, and 0 2+ in tap water samples. Such technique may complement existing biosensors to add a sensing modality to facilitate the heavy metal ions detection. Appendix A
PY - 2014/7/15
Y1 - 2014/7/15
N2 - Heavy metal ions, i.e., Cu2+, are harmful to the environment and our health. In order to detect them, and circumvent or alleviate the weaknesses of existing detecting technologies, we contrive a unique Surface Plasmon Resonance (SPR) biosensor combined with competitive adsorption of proteins, termed the Vroman effect. This approach adopts native proteins (albumin) as bio-receptors that interact with Cu2+ to be denatured. Denaturation disrupts the conformation of albumin so that it weakens its affinity to adsorb on the sensing surface. Through the competitive adsorption between the denatured albumins and the native ones, the displacement occurs adjacent to the sensing surface, and this process is real-time monitored by SPR, a surface-sensitive label-free biosensor. The affinities of native albumin is significantly higher than that of denatured albumin, demonstrated by measured KD of native and denatured albumin to gold surafce, 5.8±0.2×10-5M and 5.4±0.1×10-4M, respectively. Using our biosensor, Cu2+ with concentration down to 0.1mg/L is detected in PBS, tap water, deionized water, and bottled water. The SPR biosensor is characterized for 5 different heavy metal ions, Cu2+, Fe3+, Mn2+, Pb2+, and Hg2+, most common heavy metal ions found in tap water. At the maximum contaminant level (MCL) suggested by the United States Environmental Protection Agency (EPA), the SPR biosensor produces 13.5±0.4, 1.5±0.4, 0, 0, and 0mDeg, respectively, suggesting the biosensor may be used to detect Cu2+ in tap water samples.
AB - Heavy metal ions, i.e., Cu2+, are harmful to the environment and our health. In order to detect them, and circumvent or alleviate the weaknesses of existing detecting technologies, we contrive a unique Surface Plasmon Resonance (SPR) biosensor combined with competitive adsorption of proteins, termed the Vroman effect. This approach adopts native proteins (albumin) as bio-receptors that interact with Cu2+ to be denatured. Denaturation disrupts the conformation of albumin so that it weakens its affinity to adsorb on the sensing surface. Through the competitive adsorption between the denatured albumins and the native ones, the displacement occurs adjacent to the sensing surface, and this process is real-time monitored by SPR, a surface-sensitive label-free biosensor. The affinities of native albumin is significantly higher than that of denatured albumin, demonstrated by measured KD of native and denatured albumin to gold surafce, 5.8±0.2×10-5M and 5.4±0.1×10-4M, respectively. Using our biosensor, Cu2+ with concentration down to 0.1mg/L is detected in PBS, tap water, deionized water, and bottled water. The SPR biosensor is characterized for 5 different heavy metal ions, Cu2+, Fe3+, Mn2+, Pb2+, and Hg2+, most common heavy metal ions found in tap water. At the maximum contaminant level (MCL) suggested by the United States Environmental Protection Agency (EPA), the SPR biosensor produces 13.5±0.4, 1.5±0.4, 0, 0, and 0mDeg, respectively, suggesting the biosensor may be used to detect Cu2+ in tap water samples.
KW - Biosensor
KW - Heavy metal ions
KW - Surface plasmon resonance (SPR)
KW - Vroman effect
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UR - http://www.scopus.com/inward/citedby.url?scp=84894652983&partnerID=8YFLogxK
U2 - 10.1016/j.bios.2014.01.056
DO - 10.1016/j.bios.2014.01.056
M3 - Article
C2 - 24583689
AN - SCOPUS:84894652983
SN - 0956-5663
VL - 57
SP - 179
EP - 185
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
ER -