Detection of calcium binding by photosystem II polypeptides immobilised onto nitrocellulose membrane

Andrew Webber, John C. Gray

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Photosystem II calcium-binding polypeptides have been detected by their ability to selectively bind 45Ca when immobilised onto nitrocellulose membrane following SDS-polyacrylamide gel electrophoresis. Two calcium-binding polypeptides of 26 kDa and 24 kDa are shown to be components of LHCII. The 24 kDa polypeptide was further characterised by N-terminal amino acid sequence analysis and shown to be the product of a type II cab gene. A third polypeptide of 33 kDa bound calcium more weakly and was not positively identified.

Original languageEnglish (US)
Pages (from-to)79-82
Number of pages4
JournalFEBS Letters
Volume249
Issue number1
DOIs
StatePublished - May 22 1989
Externally publishedYes

Fingerprint

Photosystem II Protein Complex
Collodion
Calcium
Membranes
Peptides
Protein Sequence Analysis
Electrophoresis
Polyacrylamide Gel Electrophoresis
Genes
Amino Acids

Keywords

  • Calcium-binding polypeptide
  • Light-harvesting complex II
  • N-terminal sequence
  • Photosystem II

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Detection of calcium binding by photosystem II polypeptides immobilised onto nitrocellulose membrane. / Webber, Andrew; Gray, John C.

In: FEBS Letters, Vol. 249, No. 1, 22.05.1989, p. 79-82.

Research output: Contribution to journalArticle

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