Detection of calcium binding by photosystem II polypeptides immobilised onto nitrocellulose membrane

Andrew N. Webber, John C. Gray

Research output: Contribution to journalArticle

35 Scopus citations

Abstract

Photosystem II calcium-binding polypeptides have been detected by their ability to selectively bind 45Ca when immobilised onto nitrocellulose membrane following SDS-polyacrylamide gel electrophoresis. Two calcium-binding polypeptides of 26 kDa and 24 kDa are shown to be components of LHCII. The 24 kDa polypeptide was further characterised by N-terminal amino acid sequence analysis and shown to be the product of a type II cab gene. A third polypeptide of 33 kDa bound calcium more weakly and was not positively identified.

Original languageEnglish (US)
Pages (from-to)79-82
Number of pages4
JournalFEBS Letters
Volume249
Issue number1
DOIs
StatePublished - May 22 1989
Externally publishedYes

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Keywords

  • Calcium-binding polypeptide
  • Light-harvesting complex II
  • N-terminal sequence
  • Photosystem II

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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