Detection of bumetanide in an over-the-counter dietary supplement

Archie M. Hoggan, Melinda K. Shelby, Dennis J. Crouch, Chad Borges, Matthew H. Slawson

    Research output: Contribution to journalArticle

    6 Citations (Scopus)

    Abstract

    Bumetanide is a loop diuretic used clinically to treat heart failure, acute renal failure, high blood pressure, and edema. However, diuretics may also be used by athletes as masking agents and to decrease weight. Taken as masking agents, diuretics increase urine production and decrease urinary concentrations of banned performance-enhancing agents, such as anabolic steroids. StarCaps® is an over-the-counter dietary supplement marketed as a diet aid. The manufacturer claims that the product contains only natural cleansing agents and emphasizes that it is free from traditional appetite suppressants such as sympathomimetic amines. However, no such disclaimer is made concerning diuretic agents. A single StarCaps capsule was administered to two male and two female volunteers, and their urine specimens were collected at discrete intervals (2, 4, 8, and 12 h) post administration. The specimens were analyzed by a high-performance liquid chromatography-mass spectrometry quadrupole (HPLC-MS) method, and bumetanide was detected in all specimens (4.6 to 351.3 ng/mL). Adjusting the bumetanide concentrations for creatinine content did little to normalize the excretion profiles. Bumetanide was also detected in the StarCaps capsules at concentrations approaching therapeutic doses. HPLC-quadrupole-time- of-flight mass spectrometry was used to confirm the presence of bumetanide in the urine samples and StarCaps capsules. The results showed that unregulated dietary supplements may put consumers at risk for unwitting consumption of prescription medications, and that it is possible for athletes to inadvertently test positive for bumetanide and face disciplinary actions.

    Original languageEnglish (US)
    Pages (from-to)601-604
    Number of pages4
    JournalJournal of Analytical Toxicology
    Volume31
    Issue number9
    StatePublished - Nov 2007

    Fingerprint

    Dietary supplements
    Bumetanide
    Dietary Supplements
    urine
    mass spectrometry
    Diuretics
    Capsules
    Urine
    Mass spectrometry
    steroid
    excretion
    Athletes
    liquid chromatography
    aid
    Mass Spectrometry
    Testosterone Congeners
    blood
    High Pressure Liquid Chromatography
    diet
    Sodium Potassium Chloride Symporter Inhibitors

    ASJC Scopus subject areas

    • Analytical Chemistry
    • Toxicology
    • Health, Toxicology and Mutagenesis

    Cite this

    Hoggan, A. M., Shelby, M. K., Crouch, D. J., Borges, C., & Slawson, M. H. (2007). Detection of bumetanide in an over-the-counter dietary supplement. Journal of Analytical Toxicology, 31(9), 601-604.

    Detection of bumetanide in an over-the-counter dietary supplement. / Hoggan, Archie M.; Shelby, Melinda K.; Crouch, Dennis J.; Borges, Chad; Slawson, Matthew H.

    In: Journal of Analytical Toxicology, Vol. 31, No. 9, 11.2007, p. 601-604.

    Research output: Contribution to journalArticle

    Hoggan, AM, Shelby, MK, Crouch, DJ, Borges, C & Slawson, MH 2007, 'Detection of bumetanide in an over-the-counter dietary supplement', Journal of Analytical Toxicology, vol. 31, no. 9, pp. 601-604.
    Hoggan AM, Shelby MK, Crouch DJ, Borges C, Slawson MH. Detection of bumetanide in an over-the-counter dietary supplement. Journal of Analytical Toxicology. 2007 Nov;31(9):601-604.
    Hoggan, Archie M. ; Shelby, Melinda K. ; Crouch, Dennis J. ; Borges, Chad ; Slawson, Matthew H. / Detection of bumetanide in an over-the-counter dietary supplement. In: Journal of Analytical Toxicology. 2007 ; Vol. 31, No. 9. pp. 601-604.
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