Detection of bound and free IGF-1 and IGF-2 in human plasma via biomolecular interaction analysis mass spectrometry

Dobrin Nedelkov, Randall W. Nelson, Urban A. Kiernan, Eric E. Niederkofler, Kemmons A. Tubbs

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

Insulin like growth factor (IGF)-1 and IGF-2 were assayed from human plasma via biomolecular interaction analysis mass spectrometry, utilizing antibodies as ligands for affinity retrieval. Detection of both targeted and non-targeted IGFs in the mass spectra indicated possible protein complex retrieval by the individual antibodies. A series of control experiments eliminated the possibility of analyte cross-walking between flow cells, significant antibodies cross-reactivity, and direct IGF interactions. To disrupt the putative protein complex and release its constituent proteins, plasma samples were treated with detergents. An SDS-treated plasma yielded IGF signals in a different ratio than the one observed in the mass spectra from the non-treated plasma, suggesting disruption of the protein complex, and its retrieval from non-treated plasma. Novel truncated IGF-2 variant, missing its N-terminal Alanine, was detected in all mass spectra.

Original languageEnglish (US)
Pages (from-to)130-134
Number of pages5
JournalFEBS Letters
Volume536
Issue number1-3
DOIs
StatePublished - Feb 11 2003

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Keywords

  • Biomolecular interaction analysis mass spectrometry
  • IGF-1
  • IGF-2
  • Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
  • Protein complex
  • Surface plasmon resonance

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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