Design of semi-degradable hydrogels based on poly(vinyl alcohol) and poly(lactic-co-glycolic acid) for cartilage tissue engineering

Kara L. Spiller, Julianne Holloway, Megan E. Gribb, Anthony M. Lowman

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Articular cartilage damage is a persistent challenge in biomaterials and tissue engineering. Poly(vinyl alcohol) (PVA) hydrogels have shown promise as implants, but their lack of integration with surrounding cartilage prevents their utility. We sought to combine the advantages of PVA hydrogels with poly(lactic-co-glycolic acid) (PLGA) scaffolds, which have been successful in facilitating the integration of neocartilage with surrounding tissue. Through a novel double-emulsion technique, PLGA microparticles and a high level of porosity were simultaneously incorporated into PVA hydrogels. The porosity, average pore size and swelling properties of the hydrogels were controlled by varying initial processing parameters, such as the relative amounts of PLGA and solvent. Average pore sizes were in the ranged 50-100 μm. The PLGA microparticles degraded within the hydrogels over time in aqueous conditions, resulting in increases in porosity and pore size. After 4 weeks in cell culture, immature cartilage tissue filled many of the pores of the hydrogels that initially contained PLGA, and proteoglycan production was proportional to the amount of PLGA. In contrast, there was little cell attachment and no proteoglycan production in control hydrogels without PLGA. The compressive moduli of the hydrogels were similar to that of healthy cartilage and increased over time from 0.05-0.1 to 0.3-0.7 MPa. The generation of a hybrid cartilage-hydrogel construct using this technique may finally allow the integration of PVA hydrogels with surrounding cartilage.

Original languageEnglish (US)
Pages (from-to)636-647
Number of pages12
JournalJournal of Tissue Engineering and Regenerative Medicine
Volume5
Issue number8
DOIs
StatePublished - Aug 2011
Externally publishedYes

Fingerprint

Hydrogels
Cartilage
Tissue Engineering
Tissue engineering
Alcohols
Acids
Porosity
Pore size
Proteoglycans
Tissue
polylactic acid-polyglycolic acid copolymer
Milk
Hydrogel
Articular Cartilage
Biocompatible Materials
Emulsions
Cell culture
Scaffolds
Swelling
Biomaterials

Keywords

  • Cartilage
  • Hydrogel
  • Poly(lactic-co-glycolic acid)
  • Poly(vinyl alcohol)
  • Porosity
  • Swelling behaviour

ASJC Scopus subject areas

  • Biomedical Engineering
  • Medicine (miscellaneous)
  • Biomaterials

Cite this

Design of semi-degradable hydrogels based on poly(vinyl alcohol) and poly(lactic-co-glycolic acid) for cartilage tissue engineering. / Spiller, Kara L.; Holloway, Julianne; Gribb, Megan E.; Lowman, Anthony M.

In: Journal of Tissue Engineering and Regenerative Medicine, Vol. 5, No. 8, 08.2011, p. 636-647.

Research output: Contribution to journalArticle

@article{99f8ea6a5f964968a1fc9e83683010b5,
title = "Design of semi-degradable hydrogels based on poly(vinyl alcohol) and poly(lactic-co-glycolic acid) for cartilage tissue engineering",
abstract = "Articular cartilage damage is a persistent challenge in biomaterials and tissue engineering. Poly(vinyl alcohol) (PVA) hydrogels have shown promise as implants, but their lack of integration with surrounding cartilage prevents their utility. We sought to combine the advantages of PVA hydrogels with poly(lactic-co-glycolic acid) (PLGA) scaffolds, which have been successful in facilitating the integration of neocartilage with surrounding tissue. Through a novel double-emulsion technique, PLGA microparticles and a high level of porosity were simultaneously incorporated into PVA hydrogels. The porosity, average pore size and swelling properties of the hydrogels were controlled by varying initial processing parameters, such as the relative amounts of PLGA and solvent. Average pore sizes were in the ranged 50-100 μm. The PLGA microparticles degraded within the hydrogels over time in aqueous conditions, resulting in increases in porosity and pore size. After 4 weeks in cell culture, immature cartilage tissue filled many of the pores of the hydrogels that initially contained PLGA, and proteoglycan production was proportional to the amount of PLGA. In contrast, there was little cell attachment and no proteoglycan production in control hydrogels without PLGA. The compressive moduli of the hydrogels were similar to that of healthy cartilage and increased over time from 0.05-0.1 to 0.3-0.7 MPa. The generation of a hybrid cartilage-hydrogel construct using this technique may finally allow the integration of PVA hydrogels with surrounding cartilage.",
keywords = "Cartilage, Hydrogel, Poly(lactic-co-glycolic acid), Poly(vinyl alcohol), Porosity, Swelling behaviour",
author = "Spiller, {Kara L.} and Julianne Holloway and Gribb, {Megan E.} and Lowman, {Anthony M.}",
year = "2011",
month = "8",
doi = "10.1002/term.356",
language = "English (US)",
volume = "5",
pages = "636--647",
journal = "Journal of Tissue Engineering and Regenerative Medicine",
issn = "1932-6254",
publisher = "John Wiley and Sons Ltd",
number = "8",

}

TY - JOUR

T1 - Design of semi-degradable hydrogels based on poly(vinyl alcohol) and poly(lactic-co-glycolic acid) for cartilage tissue engineering

AU - Spiller, Kara L.

AU - Holloway, Julianne

AU - Gribb, Megan E.

AU - Lowman, Anthony M.

PY - 2011/8

Y1 - 2011/8

N2 - Articular cartilage damage is a persistent challenge in biomaterials and tissue engineering. Poly(vinyl alcohol) (PVA) hydrogels have shown promise as implants, but their lack of integration with surrounding cartilage prevents their utility. We sought to combine the advantages of PVA hydrogels with poly(lactic-co-glycolic acid) (PLGA) scaffolds, which have been successful in facilitating the integration of neocartilage with surrounding tissue. Through a novel double-emulsion technique, PLGA microparticles and a high level of porosity were simultaneously incorporated into PVA hydrogels. The porosity, average pore size and swelling properties of the hydrogels were controlled by varying initial processing parameters, such as the relative amounts of PLGA and solvent. Average pore sizes were in the ranged 50-100 μm. The PLGA microparticles degraded within the hydrogels over time in aqueous conditions, resulting in increases in porosity and pore size. After 4 weeks in cell culture, immature cartilage tissue filled many of the pores of the hydrogels that initially contained PLGA, and proteoglycan production was proportional to the amount of PLGA. In contrast, there was little cell attachment and no proteoglycan production in control hydrogels without PLGA. The compressive moduli of the hydrogels were similar to that of healthy cartilage and increased over time from 0.05-0.1 to 0.3-0.7 MPa. The generation of a hybrid cartilage-hydrogel construct using this technique may finally allow the integration of PVA hydrogels with surrounding cartilage.

AB - Articular cartilage damage is a persistent challenge in biomaterials and tissue engineering. Poly(vinyl alcohol) (PVA) hydrogels have shown promise as implants, but their lack of integration with surrounding cartilage prevents their utility. We sought to combine the advantages of PVA hydrogels with poly(lactic-co-glycolic acid) (PLGA) scaffolds, which have been successful in facilitating the integration of neocartilage with surrounding tissue. Through a novel double-emulsion technique, PLGA microparticles and a high level of porosity were simultaneously incorporated into PVA hydrogels. The porosity, average pore size and swelling properties of the hydrogels were controlled by varying initial processing parameters, such as the relative amounts of PLGA and solvent. Average pore sizes were in the ranged 50-100 μm. The PLGA microparticles degraded within the hydrogels over time in aqueous conditions, resulting in increases in porosity and pore size. After 4 weeks in cell culture, immature cartilage tissue filled many of the pores of the hydrogels that initially contained PLGA, and proteoglycan production was proportional to the amount of PLGA. In contrast, there was little cell attachment and no proteoglycan production in control hydrogels without PLGA. The compressive moduli of the hydrogels were similar to that of healthy cartilage and increased over time from 0.05-0.1 to 0.3-0.7 MPa. The generation of a hybrid cartilage-hydrogel construct using this technique may finally allow the integration of PVA hydrogels with surrounding cartilage.

KW - Cartilage

KW - Hydrogel

KW - Poly(lactic-co-glycolic acid)

KW - Poly(vinyl alcohol)

KW - Porosity

KW - Swelling behaviour

UR - http://www.scopus.com/inward/record.url?scp=79960547676&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79960547676&partnerID=8YFLogxK

U2 - 10.1002/term.356

DO - 10.1002/term.356

M3 - Article

VL - 5

SP - 636

EP - 647

JO - Journal of Tissue Engineering and Regenerative Medicine

JF - Journal of Tissue Engineering and Regenerative Medicine

SN - 1932-6254

IS - 8

ER -