Design and expression of a short peptide as an HIV detection probe

Jamie A. Lines, Zhiqiang Yu, Larisa Dedkova, Shengxi Chen

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

To explore a low-cost novel probe for HIV detection, we designed and prepared a 50-amino acid-length short fusion peptide (FP-50) via Escherichia coli in vivo expression. It was employed as a novel probe to detect HIV-1 gp120 protein. The detectable level of gp120 protein using the FP-50 peptide was approximately 20-200 times lower than previously published methods that used a pair of monoclonal antibodies. Thus, this short peptide is a very promising component for detection of gp120 protein during early stages of HIV infection.

Original languageEnglish (US)
Pages (from-to)308-312
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume443
Issue number1
DOIs
StatePublished - Jan 3 2014

Keywords

  • Early stages
  • Fusion peptide
  • Gp120
  • HIV detection
  • In vivo expression

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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