Description of Hymenobacter arizonensis sp. nov. from the southwestern arid lands of the United States of America

Gundlapally S N Reddy, Ferran Garcia-Pichel

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Strain OR362-8T was isolated from a biological soil crust sample collected from the southwestern arid lands of the United States of America, using BG11-PGY medium. Cells of OR362-8T were found to be rod shaped; occur singly, as pairs and in groups; non-motile; positive for catalase, oxidase, phosphatase and gelatinase; hydrolyze starch; contain iso-C 15:0, anteiso-C15:0, iso-C15:1G, C 16:1ω5c and summed feature 3 (C16:1(ω7c)/iso- C15:0 2OH as defined by the MIDI system) as the major fatty acids; and MK-7 as the sole respiratory quinone. A BLAST sequence similarity search using 16S rRNA gene sequence of OR362-8T identified Hymenobacter as the nearest genus with a similarity of 90.4-96.9 %. The phylogenetic analyses based on the phenetic methods UPGMA, NJ, ME and DNA parsimony resulted in the clustering of OR362-8T with Clade 1 Hymenobacter species represented by Hymenobacter glaciei, Hymenobacter antarcticus, Hymenobacter flocculans, Hymenobacter metalli and Hymenobacter soli with the closest being the Hymenobacter glaciei (96.9 % 16S rRNA gene sequence similarity). Besides the strong phylogentic affiliation, OR362-8T also exhibited significant phenotypic and chemotaxonomic differences with the members of Clade 1 Hymenobacter spp. More importantly, the DNA G+C content (mol%) of OR362-8 T is very high (70 %) compared to the nearest species identified by phylogenetic analysis. Based on the phylogenetic, phenotypic and chemotaxonomic characteristics, OR362-8T was assigned to a novel species for which we propose here the name Hymenobacter arizonensis sp. nov., with OR362-8 T (=ATCC BAA 1266T = DSM 17860T = JCM 13504T) as the type strain.

Original languageEnglish (US)
Pages (from-to)321-330
Number of pages10
JournalAntonie van Leeuwenhoek, International Journal of General and Molecular Microbiology
Volume103
Issue number2
DOIs
StatePublished - Feb 2013

Fingerprint

rRNA Genes
Gelatinases
DNA
Base Composition
Phosphoric Monoester Hydrolases
Starch
Catalase
Names
Cluster Analysis
Oxidoreductases
Fatty Acids
Soil
benzoquinone
C 15

Keywords

  • 16S rRNA gene sequence
  • BG11-PGY medium
  • Biological soil crusts
  • Hymenobacter arizonensis

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

@article{e161af4035914c27b7eeb107e2b4c34f,
title = "Description of Hymenobacter arizonensis sp. nov. from the southwestern arid lands of the United States of America",
abstract = "Strain OR362-8T was isolated from a biological soil crust sample collected from the southwestern arid lands of the United States of America, using BG11-PGY medium. Cells of OR362-8T were found to be rod shaped; occur singly, as pairs and in groups; non-motile; positive for catalase, oxidase, phosphatase and gelatinase; hydrolyze starch; contain iso-C 15:0, anteiso-C15:0, iso-C15:1G, C 16:1ω5c and summed feature 3 (C16:1(ω7c)/iso- C15:0 2OH as defined by the MIDI system) as the major fatty acids; and MK-7 as the sole respiratory quinone. A BLAST sequence similarity search using 16S rRNA gene sequence of OR362-8T identified Hymenobacter as the nearest genus with a similarity of 90.4-96.9 {\%}. The phylogenetic analyses based on the phenetic methods UPGMA, NJ, ME and DNA parsimony resulted in the clustering of OR362-8T with Clade 1 Hymenobacter species represented by Hymenobacter glaciei, Hymenobacter antarcticus, Hymenobacter flocculans, Hymenobacter metalli and Hymenobacter soli with the closest being the Hymenobacter glaciei (96.9 {\%} 16S rRNA gene sequence similarity). Besides the strong phylogentic affiliation, OR362-8T also exhibited significant phenotypic and chemotaxonomic differences with the members of Clade 1 Hymenobacter spp. More importantly, the DNA G+C content (mol{\%}) of OR362-8 T is very high (70 {\%}) compared to the nearest species identified by phylogenetic analysis. Based on the phylogenetic, phenotypic and chemotaxonomic characteristics, OR362-8T was assigned to a novel species for which we propose here the name Hymenobacter arizonensis sp. nov., with OR362-8 T (=ATCC BAA 1266T = DSM 17860T = JCM 13504T) as the type strain.",
keywords = "16S rRNA gene sequence, BG11-PGY medium, Biological soil crusts, Hymenobacter arizonensis",
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N2 - Strain OR362-8T was isolated from a biological soil crust sample collected from the southwestern arid lands of the United States of America, using BG11-PGY medium. Cells of OR362-8T were found to be rod shaped; occur singly, as pairs and in groups; non-motile; positive for catalase, oxidase, phosphatase and gelatinase; hydrolyze starch; contain iso-C 15:0, anteiso-C15:0, iso-C15:1G, C 16:1ω5c and summed feature 3 (C16:1(ω7c)/iso- C15:0 2OH as defined by the MIDI system) as the major fatty acids; and MK-7 as the sole respiratory quinone. A BLAST sequence similarity search using 16S rRNA gene sequence of OR362-8T identified Hymenobacter as the nearest genus with a similarity of 90.4-96.9 %. The phylogenetic analyses based on the phenetic methods UPGMA, NJ, ME and DNA parsimony resulted in the clustering of OR362-8T with Clade 1 Hymenobacter species represented by Hymenobacter glaciei, Hymenobacter antarcticus, Hymenobacter flocculans, Hymenobacter metalli and Hymenobacter soli with the closest being the Hymenobacter glaciei (96.9 % 16S rRNA gene sequence similarity). Besides the strong phylogentic affiliation, OR362-8T also exhibited significant phenotypic and chemotaxonomic differences with the members of Clade 1 Hymenobacter spp. More importantly, the DNA G+C content (mol%) of OR362-8 T is very high (70 %) compared to the nearest species identified by phylogenetic analysis. Based on the phylogenetic, phenotypic and chemotaxonomic characteristics, OR362-8T was assigned to a novel species for which we propose here the name Hymenobacter arizonensis sp. nov., with OR362-8 T (=ATCC BAA 1266T = DSM 17860T = JCM 13504T) as the type strain.

AB - Strain OR362-8T was isolated from a biological soil crust sample collected from the southwestern arid lands of the United States of America, using BG11-PGY medium. Cells of OR362-8T were found to be rod shaped; occur singly, as pairs and in groups; non-motile; positive for catalase, oxidase, phosphatase and gelatinase; hydrolyze starch; contain iso-C 15:0, anteiso-C15:0, iso-C15:1G, C 16:1ω5c and summed feature 3 (C16:1(ω7c)/iso- C15:0 2OH as defined by the MIDI system) as the major fatty acids; and MK-7 as the sole respiratory quinone. A BLAST sequence similarity search using 16S rRNA gene sequence of OR362-8T identified Hymenobacter as the nearest genus with a similarity of 90.4-96.9 %. The phylogenetic analyses based on the phenetic methods UPGMA, NJ, ME and DNA parsimony resulted in the clustering of OR362-8T with Clade 1 Hymenobacter species represented by Hymenobacter glaciei, Hymenobacter antarcticus, Hymenobacter flocculans, Hymenobacter metalli and Hymenobacter soli with the closest being the Hymenobacter glaciei (96.9 % 16S rRNA gene sequence similarity). Besides the strong phylogentic affiliation, OR362-8T also exhibited significant phenotypic and chemotaxonomic differences with the members of Clade 1 Hymenobacter spp. More importantly, the DNA G+C content (mol%) of OR362-8 T is very high (70 %) compared to the nearest species identified by phylogenetic analysis. Based on the phylogenetic, phenotypic and chemotaxonomic characteristics, OR362-8T was assigned to a novel species for which we propose here the name Hymenobacter arizonensis sp. nov., with OR362-8 T (=ATCC BAA 1266T = DSM 17860T = JCM 13504T) as the type strain.

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