Description of Deinococcus oregonensis sp. nov., from biological soil crusts in the Southwestern arid lands of the United States of America

Sathyanarayana Reddy Gundlapally, Ferran Garcia-Pichel

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Biological soil crusts are distinct habitats, harbor unique prokaryotic diversity and gave an impetus to isolate novel species. In the present study, a pink-pigmented bacterium, (OR316-6T), was isolated from biological soil crusts using oligotrophic BG11-PGY medium. Strain OR316-6T was Gram-positive, short rods, non-motile and non-spore forming. Cells were positive for catalase, oxidase and β-galactosidase and negative for most of the enzymatic activities. The major fatty acids present were C16:0, C17:0, and C16:1ω7c and contained MK-8 and MK-10 as the predominant menaquinones. The cell wall peptidoglycan was of A3β variant with l-ornithine as the diamino acid. Based on the above characteristics, strain OR316-6T was assigned to the genus Deinococcus. The phylogenetic analysis indicated that strain OR316-6T was closely related to D. aquatilis DSM 23025T with a 16S rRNA gene similarity of 99.3 % and clustered with a bootstrap value of 100 %. DNA–DNA similarity between strain OR316-6T and D. aquatilis DSM 23025T was 37.0 % indicating that strain OR316-6T was a novel species. Further, DNA fingerprinting of stains OR316-6T and D. aquatilis DSM 23035T demonstrated that both strains were related to each other with a similarity coefficient of only 0.32 and supported the species status to strain OR316-6T. In addition, phenotypic characteristics distinguished strain OR316-6T from D. aquatilis DSM 23025T. Based on the cumulative differences, strain OR316-16T exhibited with its closely related species, it was identified as a novel species and proposed the name Deinococcus oregonensis sp. nov. The type strain is D. oregonensis sp. nov. (OR316-6T = JCM 13503T = DSM 17762T).

Original languageEnglish (US)
Pages (from-to)1-8
Number of pages8
JournalArchives of Microbiology
DOIs
StateAccepted/In press - Aug 11 2016

Fingerprint

Deinococcus
Soil
Gram-Positive Rods
Galactosidases
Soils
Vitamin K 2
Ornithine
DNA Fingerprinting
Peptidoglycan
rRNA Genes
Catalase
Cell Wall
Names
Ecosystem
Oxidoreductases
Coloring Agents
Fatty Acids
Bacteria
Acids
Ports and harbors

Keywords

  • Biological soil crusts
  • Deinococcus oregonensis

ASJC Scopus subject areas

  • Microbiology
  • Biochemistry
  • Medicine(all)
  • Molecular Biology
  • Genetics

Cite this

@article{a6174933fbd5430b84db1db514be4090,
title = "Description of Deinococcus oregonensis sp. nov., from biological soil crusts in the Southwestern arid lands of the United States of America",
abstract = "Biological soil crusts are distinct habitats, harbor unique prokaryotic diversity and gave an impetus to isolate novel species. In the present study, a pink-pigmented bacterium, (OR316-6T), was isolated from biological soil crusts using oligotrophic BG11-PGY medium. Strain OR316-6T was Gram-positive, short rods, non-motile and non-spore forming. Cells were positive for catalase, oxidase and β-galactosidase and negative for most of the enzymatic activities. The major fatty acids present were C16:0, C17:0, and C16:1ω7c and contained MK-8 and MK-10 as the predominant menaquinones. The cell wall peptidoglycan was of A3β variant with l-ornithine as the diamino acid. Based on the above characteristics, strain OR316-6T was assigned to the genus Deinococcus. The phylogenetic analysis indicated that strain OR316-6T was closely related to D. aquatilis DSM 23025T with a 16S rRNA gene similarity of 99.3 {\%} and clustered with a bootstrap value of 100 {\%}. DNA–DNA similarity between strain OR316-6T and D. aquatilis DSM 23025T was 37.0 {\%} indicating that strain OR316-6T was a novel species. Further, DNA fingerprinting of stains OR316-6T and D. aquatilis DSM 23035T demonstrated that both strains were related to each other with a similarity coefficient of only 0.32 and supported the species status to strain OR316-6T. In addition, phenotypic characteristics distinguished strain OR316-6T from D. aquatilis DSM 23025T. Based on the cumulative differences, strain OR316-16T exhibited with its closely related species, it was identified as a novel species and proposed the name Deinococcus oregonensis sp. nov. The type strain is D. oregonensis sp. nov. (OR316-6T = JCM 13503T = DSM 17762T).",
keywords = "Biological soil crusts, Deinococcus oregonensis",
author = "Gundlapally, {Sathyanarayana Reddy} and Ferran Garcia-Pichel",
year = "2016",
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doi = "10.1007/s00203-016-1273-x",
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T1 - Description of Deinococcus oregonensis sp. nov., from biological soil crusts in the Southwestern arid lands of the United States of America

AU - Gundlapally, Sathyanarayana Reddy

AU - Garcia-Pichel, Ferran

PY - 2016/8/11

Y1 - 2016/8/11

N2 - Biological soil crusts are distinct habitats, harbor unique prokaryotic diversity and gave an impetus to isolate novel species. In the present study, a pink-pigmented bacterium, (OR316-6T), was isolated from biological soil crusts using oligotrophic BG11-PGY medium. Strain OR316-6T was Gram-positive, short rods, non-motile and non-spore forming. Cells were positive for catalase, oxidase and β-galactosidase and negative for most of the enzymatic activities. The major fatty acids present were C16:0, C17:0, and C16:1ω7c and contained MK-8 and MK-10 as the predominant menaquinones. The cell wall peptidoglycan was of A3β variant with l-ornithine as the diamino acid. Based on the above characteristics, strain OR316-6T was assigned to the genus Deinococcus. The phylogenetic analysis indicated that strain OR316-6T was closely related to D. aquatilis DSM 23025T with a 16S rRNA gene similarity of 99.3 % and clustered with a bootstrap value of 100 %. DNA–DNA similarity between strain OR316-6T and D. aquatilis DSM 23025T was 37.0 % indicating that strain OR316-6T was a novel species. Further, DNA fingerprinting of stains OR316-6T and D. aquatilis DSM 23035T demonstrated that both strains were related to each other with a similarity coefficient of only 0.32 and supported the species status to strain OR316-6T. In addition, phenotypic characteristics distinguished strain OR316-6T from D. aquatilis DSM 23025T. Based on the cumulative differences, strain OR316-16T exhibited with its closely related species, it was identified as a novel species and proposed the name Deinococcus oregonensis sp. nov. The type strain is D. oregonensis sp. nov. (OR316-6T = JCM 13503T = DSM 17762T).

AB - Biological soil crusts are distinct habitats, harbor unique prokaryotic diversity and gave an impetus to isolate novel species. In the present study, a pink-pigmented bacterium, (OR316-6T), was isolated from biological soil crusts using oligotrophic BG11-PGY medium. Strain OR316-6T was Gram-positive, short rods, non-motile and non-spore forming. Cells were positive for catalase, oxidase and β-galactosidase and negative for most of the enzymatic activities. The major fatty acids present were C16:0, C17:0, and C16:1ω7c and contained MK-8 and MK-10 as the predominant menaquinones. The cell wall peptidoglycan was of A3β variant with l-ornithine as the diamino acid. Based on the above characteristics, strain OR316-6T was assigned to the genus Deinococcus. The phylogenetic analysis indicated that strain OR316-6T was closely related to D. aquatilis DSM 23025T with a 16S rRNA gene similarity of 99.3 % and clustered with a bootstrap value of 100 %. DNA–DNA similarity between strain OR316-6T and D. aquatilis DSM 23025T was 37.0 % indicating that strain OR316-6T was a novel species. Further, DNA fingerprinting of stains OR316-6T and D. aquatilis DSM 23035T demonstrated that both strains were related to each other with a similarity coefficient of only 0.32 and supported the species status to strain OR316-6T. In addition, phenotypic characteristics distinguished strain OR316-6T from D. aquatilis DSM 23025T. Based on the cumulative differences, strain OR316-16T exhibited with its closely related species, it was identified as a novel species and proposed the name Deinococcus oregonensis sp. nov. The type strain is D. oregonensis sp. nov. (OR316-6T = JCM 13503T = DSM 17762T).

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