TY - JOUR
T1 - Dermal fibroblast and epidermal keratinocyte functionality on titania nanotube arrays
AU - Smith, Barbara S.
AU - Yoriya, Sorachon
AU - Johnson, Thomas
AU - Popat, Ketul C.
N1 - Funding Information:
Funding support for this work was provided by the National Science Foundation ( CBET 0827827 ) and the Colorado Office of Economic Development and International Trade . The authors would like to thank Benjamin Bird at Colorado State University for his assistance with the statistical analysis; and Dr. Craig A. Grimes at The Pennsylvania State University for his assistance with the fabrication of the titania nanotube arrays.
PY - 2011/6
Y1 - 2011/6
N2 - Transcutaneous implants that penetrate through the depth of the skin are used in numerous clinical applications, including prosthetics and dental implants. Favorable interactions between the implant surface and the respective skin layers are critical for the long-term success of transcutaneous implantable devices, hence, it is essential to understand the physiologic response elicited by skin-biomaterial interactions. Recent studies have shown that material surfaces that provide topographic cues at the nanoscale level may provide one possible solution to enhanced biomaterial integration, thus preventing biomaterial rejection. In this study titania nanotube arrays were fabricated using a simple anodization technique as potential interfaces for transcutaneous implantable devices. The in vitro functionality of human dermal fibroblasts and epidermal keratinocytes were evaluated on these nanotube arrays (diameter 70-90 nm, length 1-1.5 μm). Cellular functionality in terms of adhesion, proliferation, orientation, viability, cytoskeletal organization, differentiation and morphology were investigated for up to 4 days in culture using fluorescence microscope imaging, a cell viability assay, indirect immunofluorescence and scanning electron microscopy. The results reported in this study indicate increased dermal fibroblast and decreased epidermal keratinocyte adhesion, proliferation and differentiation on titania nanotube arrays.
AB - Transcutaneous implants that penetrate through the depth of the skin are used in numerous clinical applications, including prosthetics and dental implants. Favorable interactions between the implant surface and the respective skin layers are critical for the long-term success of transcutaneous implantable devices, hence, it is essential to understand the physiologic response elicited by skin-biomaterial interactions. Recent studies have shown that material surfaces that provide topographic cues at the nanoscale level may provide one possible solution to enhanced biomaterial integration, thus preventing biomaterial rejection. In this study titania nanotube arrays were fabricated using a simple anodization technique as potential interfaces for transcutaneous implantable devices. The in vitro functionality of human dermal fibroblasts and epidermal keratinocytes were evaluated on these nanotube arrays (diameter 70-90 nm, length 1-1.5 μm). Cellular functionality in terms of adhesion, proliferation, orientation, viability, cytoskeletal organization, differentiation and morphology were investigated for up to 4 days in culture using fluorescence microscope imaging, a cell viability assay, indirect immunofluorescence and scanning electron microscopy. The results reported in this study indicate increased dermal fibroblast and decreased epidermal keratinocyte adhesion, proliferation and differentiation on titania nanotube arrays.
KW - Cell differentiation
KW - Human dermal fibroblasts
KW - Human epidermal keratinocytes
KW - Titania nanotube arrays
KW - Transcutaneous implants
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U2 - 10.1016/j.actbio.2011.03.014
DO - 10.1016/j.actbio.2011.03.014
M3 - Article
C2 - 21414425
AN - SCOPUS:79955612414
SN - 1742-7061
VL - 7
SP - 2686
EP - 2696
JO - Acta Biomaterialia
JF - Acta Biomaterialia
IS - 6
ER -