@article{538466f8f770414dafce5ed4e4155fa7,
title = "Depletion of a toxoplasma porin leads to defects in mitochondrial morphology and contacts with the endoplasmic reticulum",
abstract = "The voltage-dependent anion channel (VDAC) is a ubiquitous channel in the outer membrane of the mitochondrion with multiple roles in protein, metabolite and small molecule transport. In mammalian cells, VDAC protein, as part of a larger complex including the inositol triphosphate receptor, has been shown to have a role in mediating contacts between the mitochondria and endoplasmic reticulum (ER). We identify VDAC of the pathogenic apicomplexan Toxoplasma gondii and demonstrate its importance for parasite growth. We show that VDAC is involved in protein import and metabolite transfer to mitochondria. Further, depletion of VDAC resulted in significant morphological changes in the mitochondrion and ER, suggesting a role in mediating contacts between these organelles in T. gondii. This article has an associated First Person interview with the first author of the paper.",
keywords = "Ca, ER, Membrane contact sites, Mitochondria, Mitochondrion, Motility, Porin, Toxoplasma, VDAC",
author = "Natalia Mallo and Jana Ovciarikova and Martins-Duarte, {Erica S.} and Baehr, {Stephan C.} and Marco Biddau and Wilde, {Mary Louise} and Uboldi, {Alessandro D.} and Leandro Lemgruber and Tonkin, {Christopher J.} and Wideman, {Jeremy G.} and Harding, {Clare R.} and Lilach Sheiner",
note = "Funding Information: Research Council in Australia [GNT1183496]. M.-L.W. is supported by an Australian Research Training Program Scholarship. J.G.W. is supported by a start-up grant from the School of Life Sciences and College of Liberal Arts and Sciences at Arizona State University. Deposited in PMC for immediate release. Funding Information: This work is supported by a Biotechnology and Biological Sciences Research Council grant [BB/N003675/1] and a Wellcome Trust Investigator Award [217173/Z/ 19/Z] to L.S. L.S is a Royal Society of Edinburgh Personal Research Fellow. C.R.H. holds a Sir Henry Dale fellowship from the Wellcome Trust and Royal Society [213455/Z/18/Z] and a Lord Kelvin/Adam Smith (LKAS) Fellowship from the University of Glasgow. C.J.T. is supported by the National Health and Medical Publisher Copyright: {\textcopyright} 2021 Company of Biologists Ltd. All rights reserved.",
year = "2021",
month = oct,
doi = "10.1242/jcs.255299",
language = "English (US)",
volume = "134",
journal = "Journal of Cell Science",
issn = "0021-9533",
publisher = "Company of Biologists Ltd",
number = "20",
}