Dependence of the staphylococcal volatilome composition on microbial nutrition

Carrie L. Jenkins, Heather D. Bean

Research output: Contribution to journalArticlepeer-review

Abstract

In vitro cultivation of staphylococci is fundamental to both clinical and research microbiology, but few studies, to-date, have investigated how the differences in rich media can influence the volatilome of cultivated bacteria. The objective of this study was to determine the influence of rich media composition on the chemical characteristics of the volatilomes of Staphylococcus aureus and Staphylococcus epidermidis. S. aureus (ATCC 12600) and S. epidermidis (ATCC 12228) were cultured in triplicate in four rich complex media (brain heart infusion (BHI), lysogeny broth (LB), Mueller Hinton broth (MHB), and tryptic soy broth (TSB)), and the volatile metabolites produced by each culture were analyzed using headspace solid-phase microextraction combined with comprehensive two-dimensional gas chromatography—time-of-flight mass spectrometry (HS-SPME-GC×GC-TOFMS). When comparing the chemical compositions of the staph volatilomes by the presence versus absence of volatiles produced in each medium, we observed few differences. However, when the relative abundances of volatiles were included in the analyses, we observed that culturing staph in media containing free glucose (BHI and TSB) resulted in volatilomes dominated by acids and esters (67%). The low-glucose media (LB and MHB) produced ketones in greatest relative abundances, but the volatilome compositions in these two media were highly dissimilar. We conclude that the staphylococcal volatilome is strongly influenced by the nutritional composition of the growth medium, especially the availability of free glucose, which is much more evident when the relative abundances of the volatiles are analyzed, compared to the presence versus absence.

Original languageEnglish (US)
Article number347
Pages (from-to)1-15
Number of pages15
JournalMetabolites
Volume10
Issue number9
DOIs
StatePublished - Sep 2020

Keywords

  • Catabolite repression control
  • GC×GC-TOFMS
  • Staphylococcus aureus
  • Staphylococcus epidermidis
  • Volatile organic compounds

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Molecular Biology

Fingerprint Dive into the research topics of 'Dependence of the staphylococcal volatilome composition on microbial nutrition'. Together they form a unique fingerprint.

Cite this