Degradation of DNA by nucleases in intestinal tract of rats

Larry Maturin, Roy Curtiss

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Strains of Escherichia coli K12 have been constructed as safer hosts for use in recombinant DNA research. These strains are unable to survive passage through the intestinal tracts of rats because of a constellation of mutations conferring bile sensitivity and requirements for diaminopimelic acid and thymine. Since death caused by diaminopimelic acid deprivation could release recombinant DNA before DNA is degraded because of thymine starvation, it is important to determine the "survival potential" of the released DNA's. Bacterial and plasmid DNA's extracted from bacterial cells are rapidly degraded when added to low dilutions of rat intestinal contents. This observation, coupled with the stringent requirements necessary for in vitro transformation or transfection, make in vivo transmission of naked recombinant DNA in the rat intestinal tract highly improbable.

Original languageEnglish (US)
Pages (from-to)216-218
Number of pages3
JournalScience
Volume196
Issue number4286
StatePublished - 1977
Externally publishedYes

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Recombinant DNA
Deoxyribonucleases
Diaminopimelic Acid
Thymine
Escherichia coli K12
Bacterial DNA
Gastrointestinal Contents
DNA
Starvation
Bile
Transfection
Plasmids
Mutation

ASJC Scopus subject areas

  • General

Cite this

Maturin, L., & Curtiss, R. (1977). Degradation of DNA by nucleases in intestinal tract of rats. Science, 196(4286), 216-218.

Degradation of DNA by nucleases in intestinal tract of rats. / Maturin, Larry; Curtiss, Roy.

In: Science, Vol. 196, No. 4286, 1977, p. 216-218.

Research output: Contribution to journalArticle

Maturin, L & Curtiss, R 1977, 'Degradation of DNA by nucleases in intestinal tract of rats', Science, vol. 196, no. 4286, pp. 216-218.
Maturin L, Curtiss R. Degradation of DNA by nucleases in intestinal tract of rats. Science. 1977;196(4286):216-218.
Maturin, Larry ; Curtiss, Roy. / Degradation of DNA by nucleases in intestinal tract of rats. In: Science. 1977 ; Vol. 196, No. 4286. pp. 216-218.
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