Degradation of an extracellular matrix: sea urchin hatching enzyme removes cortical granule-derived proteins from the fertilization envelope

Nancy M. Mozingo, Lisa R. Hollar, Douglas E. Chandler

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

The sea urchin fertilization envelope is an extracellular matrix assembled at fertilization to prevent polyspermy and protect the embryo during early development. During hatching, the embryo secretes a proteolytic hatching enzyme which dissolves the fertilization envelope, allowing a ciliated blastula to swim free. In this study we examined ultrastructural changes in the fertilization envelope during degradation of this matrix by hatching enzyme. The completed fertilization envelope is a trilaminar structure consisting of a dense, central layer of filaments sandwiched between surface coats of paracrystalline material. Hatching enzyme disassembles this matrix by degrading the paracrystalline layers and removing macromolecules from the central layer leaving behind a thin matrix of loosely woven fibers.

Original languageEnglish (US)
Pages (from-to)929-938
Number of pages10
JournalJournal of Cell Science
Volume104
Issue number3
StatePublished - Jan 1 1993

Keywords

  • Fertilization envelope
  • Freeze-fracture
  • Hatching
  • Lytechinus pictus
  • Sea urchin eggs
  • Strongylocentrotus purpuratus

ASJC Scopus subject areas

  • Cell Biology

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