DEEP-well microfluidics for arrayed colorimetric LAMP analysis

Rhett L. Martineau, Shufang Ci, Jeff Houkal, Weimin Gao, Shih-Hui Chao, Deirdre Meldrum

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper presents a microfluidic device for colorimetric loop-mediated isothermal amplification (LAMP) detection based on hydroxynapthol blue (HNB), long optical pathlength reaction wells, and two-phase liquid loading into a novel array geometry. We show that an inexpensive camera can be used with diffuse illumination to detect colorimetric changes due to LAMP reactions in sub-microliter wells.

Original languageEnglish (US)
Title of host publication18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
PublisherChemical and Biological Microsystems Society
Pages1009-1011
Number of pages3
ISBN (Print)9780979806476
StatePublished - 2014
Event18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States
Duration: Oct 26 2014Oct 30 2014

Other

Other18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
CountryUnited States
CitySan Antonio
Period10/26/1410/30/14

Keywords

  • Colorimetry
  • DNA analysis
  • Isothermal amplification
  • Multiphase systems

ASJC Scopus subject areas

  • Control and Systems Engineering

Fingerprint Dive into the research topics of 'DEEP-well microfluidics for arrayed colorimetric LAMP analysis'. Together they form a unique fingerprint.

  • Cite this

    Martineau, R. L., Ci, S., Houkal, J., Gao, W., Chao, S-H., & Meldrum, D. (2014). DEEP-well microfluidics for arrayed colorimetric LAMP analysis. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 (pp. 1009-1011). Chemical and Biological Microsystems Society.