Abstract
This paper presents a microfluidic device for colorimetric loop-mediated isothermal amplification (LAMP) detection based on hydroxynapthol blue (HNB), long optical pathlength reaction wells, and two-phase liquid loading into a novel array geometry. We show that an inexpensive camera can be used with diffuse illumination to detect colorimetric changes due to LAMP reactions in sub-microliter wells.
| Original language | English (US) |
|---|---|
| Title of host publication | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 1009-1011 |
| Number of pages | 3 |
| ISBN (Print) | 9780979806476 |
| State | Published - 2014 |
| Event | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States Duration: Oct 26 2014 → Oct 30 2014 |
Other
| Other | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 |
|---|---|
| Country/Territory | United States |
| City | San Antonio |
| Period | 10/26/14 → 10/30/14 |
Keywords
- Colorimetry
- DNA analysis
- Isothermal amplification
- Multiphase systems
ASJC Scopus subject areas
- Control and Systems Engineering