DEEP-well microfluidics for arrayed colorimetric LAMP analysis

Rhett L. Martineau, Shufang Ci, Jeff Houkal, Weimin Gao, Shih-Hui Chao, Deirdre Meldrum

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper presents a microfluidic device for colorimetric loop-mediated isothermal amplification (LAMP) detection based on hydroxynapthol blue (HNB), long optical pathlength reaction wells, and two-phase liquid loading into a novel array geometry. We show that an inexpensive camera can be used with diffuse illumination to detect colorimetric changes due to LAMP reactions in sub-microliter wells.

Original languageEnglish (US)
Title of host publication18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
PublisherChemical and Biological Microsystems Society
Pages1009-1011
Number of pages3
ISBN (Print)9780979806476
StatePublished - 2014
Event18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States
Duration: Oct 26 2014Oct 30 2014

Other

Other18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
CountryUnited States
CitySan Antonio
Period10/26/1410/30/14

Fingerprint

Microfluidics
Amplification
Lighting
Cameras
Geometry
Liquids

Keywords

  • Colorimetry
  • DNA analysis
  • Isothermal amplification
  • Multiphase systems

ASJC Scopus subject areas

  • Control and Systems Engineering

Cite this

Martineau, R. L., Ci, S., Houkal, J., Gao, W., Chao, S-H., & Meldrum, D. (2014). DEEP-well microfluidics for arrayed colorimetric LAMP analysis. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 (pp. 1009-1011). Chemical and Biological Microsystems Society.

DEEP-well microfluidics for arrayed colorimetric LAMP analysis. / Martineau, Rhett L.; Ci, Shufang; Houkal, Jeff; Gao, Weimin; Chao, Shih-Hui; Meldrum, Deirdre.

18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, 2014. p. 1009-1011.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Martineau, RL, Ci, S, Houkal, J, Gao, W, Chao, S-H & Meldrum, D 2014, DEEP-well microfluidics for arrayed colorimetric LAMP analysis. in 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, pp. 1009-1011, 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, San Antonio, United States, 10/26/14.
Martineau RL, Ci S, Houkal J, Gao W, Chao S-H, Meldrum D. DEEP-well microfluidics for arrayed colorimetric LAMP analysis. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society. 2014. p. 1009-1011
Martineau, Rhett L. ; Ci, Shufang ; Houkal, Jeff ; Gao, Weimin ; Chao, Shih-Hui ; Meldrum, Deirdre. / DEEP-well microfluidics for arrayed colorimetric LAMP analysis. 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, 2014. pp. 1009-1011
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