The popularity of ebooks does not extend to an increasing interest in etextbooks among students. A common form of etextbooks is the digital replica of the original printed book; however, a lack of interactivity was considered as an issue of etextbook among college students. To explore the potential beneficial interactive affordances, this study proposes a web-based interactive etextbook with built-in multimedia videos and self-test quizzes. Assessments have been conducted to examine the effect of using the interactive etextbook on learning and perception of learning. The results imply that a combination of multiple interactive features in etextbooks could produce optimal perception on learning and show the potential for impacting learning.