TY - JOUR
T1 - Deciphering Underlying Drivers of Disease Suppressiveness Against Pathogenic Fusarium oxysporum
AU - Ou, Yannan
AU - Penton, C. Ryan
AU - Geisen, Stefan
AU - Shen, Zongzhuan
AU - Sun, Yifei
AU - Lv, Nana
AU - Wang, Beibei
AU - Ruan, Yunze
AU - Xiong, Wu
AU - Li, Rong
AU - Shen, Qirong
N1 - Funding Information:
We thank the banana orchard owner Mr. Yusheng Li for providing access to the experimental facilities in Hainan Province, China. Funding. This work was supported by the National Natural Science Foundation of China (31572212, 31601836, and 31672239), the National Key Basic Research Program of China (2015CB150500), the Science and Technology Planning Project of Guangdong Province, China (2016B020202006), the Priority Academic Program Development of the Jiangsu Higher Education Institutions (PAPD), the 111 project (B12009), Top-notch Academic Programs Project of Jiangsu Higher Education Institutions (PPZY2015A061), the Innovative Research Team Development Plan of the Ministry of Education of China (IRT_17R56), and the China Scholarship Council (award to RL for 1 year’s abroad study). SG was supported by a NWO-VENI grant from Netherlands Organisation for Scientific Research (016.Veni.181.078).
Publisher Copyright:
© Copyright © 2019 Ou, Penton, Geisen, Shen, Sun, Lv, Wang, Ruan, Xiong, Li and Shen.
PY - 2019/11/12
Y1 - 2019/11/12
N2 - Soil-borne diseases, especially those caused by fungal pathogens, lead to profound annual yield losses. One key example for such a disease is Fusarium wilt disease in banana. In some soils, plants do not show disease symptoms, even if the disease-causing pathogens are present. However, the underlying agents that make soils suppressive against Fusarium wilt remain elusive. In this study, we aimed to determine the underlying microbial agents governing soil disease-suppressiveness. We traced the shift of microbiomes during the invasion of disease-causing Fusarium oxysporum f. sp. cubense in disease-suppressive and disease-conducive soils. We found distinct microbiome structures in the suppressive and conducive soils after pathogen invasion. The alpha diversity indices increased (or did not significantly change) and decreased, respectively, in the suppressive and conducive soils, indicating that the shift pattern of the microbiome with pathogen invasion was notably different between the suppressive and conductive soils. Microbiome networks were more complex with higher numbers of links and revealed more negative links, especially between bacterial taxa and the disease-causing Fusarium, in suppressive soils than in conducive soils. We identified the bacterial genera Chryseolinea, Terrimonas, and Ohtaekwangia as key groups that likely confer suppressiveness against disease-causing Fusarium. Overall, our study provides the first insights into agents potentially underlying the disease suppressiveness of soils against Fusarium wilt pathogen invasion. The results of this study may help to guide efforts for targeted cultivation and application of these potential biocontrol agents, which might lead to the development of effective biocontrol agents against Fusarium wilt disease.
AB - Soil-borne diseases, especially those caused by fungal pathogens, lead to profound annual yield losses. One key example for such a disease is Fusarium wilt disease in banana. In some soils, plants do not show disease symptoms, even if the disease-causing pathogens are present. However, the underlying agents that make soils suppressive against Fusarium wilt remain elusive. In this study, we aimed to determine the underlying microbial agents governing soil disease-suppressiveness. We traced the shift of microbiomes during the invasion of disease-causing Fusarium oxysporum f. sp. cubense in disease-suppressive and disease-conducive soils. We found distinct microbiome structures in the suppressive and conducive soils after pathogen invasion. The alpha diversity indices increased (or did not significantly change) and decreased, respectively, in the suppressive and conducive soils, indicating that the shift pattern of the microbiome with pathogen invasion was notably different between the suppressive and conductive soils. Microbiome networks were more complex with higher numbers of links and revealed more negative links, especially between bacterial taxa and the disease-causing Fusarium, in suppressive soils than in conducive soils. We identified the bacterial genera Chryseolinea, Terrimonas, and Ohtaekwangia as key groups that likely confer suppressiveness against disease-causing Fusarium. Overall, our study provides the first insights into agents potentially underlying the disease suppressiveness of soils against Fusarium wilt pathogen invasion. The results of this study may help to guide efforts for targeted cultivation and application of these potential biocontrol agents, which might lead to the development of effective biocontrol agents against Fusarium wilt disease.
KW - Fusarium oxysporum
KW - disease-conducive soil
KW - disease-suppressive soil
KW - invasion resistance
KW - microbiome
UR - http://www.scopus.com/inward/record.url?scp=85075658483&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85075658483&partnerID=8YFLogxK
U2 - 10.3389/fmicb.2019.02535
DO - 10.3389/fmicb.2019.02535
M3 - Article
AN - SCOPUS:85075658483
SN - 1664-302X
VL - 10
JO - Frontiers in Microbiology
JF - Frontiers in Microbiology
M1 - 2535
ER -