TY - JOUR
T1 - The interior of the cell
T2 - A complex solvent. Biochemical reactions under physiological and extreme conditions
AU - Heyden, Matthias
AU - Ebbinghaus, Simon
AU - Winter, Roland
N1 - Publisher Copyright:
© 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
PY - 2017/2/1
Y1 - 2017/2/1
N2 - The interior of a living cell is very distinct from dilute aqueous solutions that are often used to study the function of proteins and enzymes experimentally. Here, we discuss novel experimental techniques and modeling approaches that provide a deeper understanding of the effects of crowding, intermolecular interactions, and the influence of osmolytes in realistic biological environments, including cells. Further, we discuss adaptation mechanisms involving in-cell solvation environments to extreme conditions, such as high pressure in the deep sea, and their relevance to biotechnology.
AB - The interior of a living cell is very distinct from dilute aqueous solutions that are often used to study the function of proteins and enzymes experimentally. Here, we discuss novel experimental techniques and modeling approaches that provide a deeper understanding of the effects of crowding, intermolecular interactions, and the influence of osmolytes in realistic biological environments, including cells. Further, we discuss adaptation mechanisms involving in-cell solvation environments to extreme conditions, such as high pressure in the deep sea, and their relevance to biotechnology.
UR - http://www.scopus.com/inward/record.url?scp=85011663040&partnerID=8YFLogxK
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U2 - 10.1002/ciuz.201700777
DO - 10.1002/ciuz.201700777
M3 - Article
AN - SCOPUS:85011663040
SN - 0009-2851
VL - 51
SP - 26
EP - 33
JO - Chemie in Unserer Zeit
JF - Chemie in Unserer Zeit
IS - 1
ER -