Cytoskeletal reorganization during early mammalian development: Analysis using embedment-free sections

David Capco, Robert W. McGaughey

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

We have examined cytoskeletal reorganization during early embryonic development in the hamster by employing detergent extraction to remove soluble components of the embryos and reveal the underlying structural network. This procedure allows examination of both the cortical cytoskeleton and the cytoskeleton of the egg interior. Sections of eggs and embryos were prepared for transmission electron microscopy with the removable embedding medium, diethylene glycol disterate which allows thicker sections than conventional embedment procedures thereby providing more spatial cues for studying organization. The cytoskeleton reorganizes after fertilization, at the time of compaction and again at the blastocyst stage. These cytoskeletal reorganizations are considered in terms of the blastomere polarity hypothesis and the involvement of the cytoskeleton with early embryonic development.

Original languageEnglish (US)
Pages (from-to)446-458
Number of pages13
JournalDevelopmental Biology
Volume115
Issue number2
DOIs
StatePublished - 1986

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Cytoskeleton
Embryonic Development
Embryonic Structures
Blastomeres
Blastocyst
Transmission Electron Microscopy
Fertilization
Cricetinae
Detergents
Eggs
Cues
Ovum

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Cytoskeletal reorganization during early mammalian development : Analysis using embedment-free sections. / Capco, David; McGaughey, Robert W.

In: Developmental Biology, Vol. 115, No. 2, 1986, p. 446-458.

Research output: Contribution to journalArticle

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