TY - JOUR
T1 - Cytoskeletal reorganization during early mammalian development
T2 - Analysis using embedment-free sections
AU - Capco, David
AU - McGaughey, Robert W.
N1 - Funding Information:
We thank Jim Swafford for suggesting the use of LR White resin, Greg Hendricks for assisting with negative staining, and Charles Ka-zilek for printing some micrographs. This work was supported by NIH Grants HD 19067 and HD 16788, by the Biomedical Research Support Grant SO7 RR07112 to Arizona State University, and by Faculty Grant-in-Aid 425069. David G. Capco is a recipient of NIH Research Career Development Award HD 00598.
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1986/6
Y1 - 1986/6
N2 - We have examined cytoskeletal reorganization during early embryonic development in the hamster by employing detergent extraction to remove soluble components of the embryos and reveal the underlying structural network. This procedure allows examination of both the cortical cytoskeleton and the cytoskeleton of the egg interior. Sections of eggs and embryos were prepared for transmission electron microscopy with the removable embedding medium, diethylene glycol disterate which allows thicker sections than conventional embedment procedures thereby providing more spatial cues for studying organization. The cytoskeleton reorganizes after fertilization, at the time of compaction and again at the blastocyst stage. These cytoskeletal reorganizations are considered in terms of the blastomere polarity hypothesis and the involvement of the cytoskeleton with early embryonic development.
AB - We have examined cytoskeletal reorganization during early embryonic development in the hamster by employing detergent extraction to remove soluble components of the embryos and reveal the underlying structural network. This procedure allows examination of both the cortical cytoskeleton and the cytoskeleton of the egg interior. Sections of eggs and embryos were prepared for transmission electron microscopy with the removable embedding medium, diethylene glycol disterate which allows thicker sections than conventional embedment procedures thereby providing more spatial cues for studying organization. The cytoskeleton reorganizes after fertilization, at the time of compaction and again at the blastocyst stage. These cytoskeletal reorganizations are considered in terms of the blastomere polarity hypothesis and the involvement of the cytoskeleton with early embryonic development.
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U2 - 10.1016/0012-1606(86)90265-4
DO - 10.1016/0012-1606(86)90265-4
M3 - Article
C2 - 3519319
AN - SCOPUS:0022507295
VL - 115
SP - 446
EP - 458
JO - Developmental Biology
JF - Developmental Biology
SN - 0012-1606
IS - 2
ER -