Cytoskeletal reorganization during early mammalian development: Analysis using embedment-free sections

David Capco, Robert W. McGaughey

Research output: Contribution to journalArticle

40 Scopus citations

Abstract

We have examined cytoskeletal reorganization during early embryonic development in the hamster by employing detergent extraction to remove soluble components of the embryos and reveal the underlying structural network. This procedure allows examination of both the cortical cytoskeleton and the cytoskeleton of the egg interior. Sections of eggs and embryos were prepared for transmission electron microscopy with the removable embedding medium, diethylene glycol disterate which allows thicker sections than conventional embedment procedures thereby providing more spatial cues for studying organization. The cytoskeleton reorganizes after fertilization, at the time of compaction and again at the blastocyst stage. These cytoskeletal reorganizations are considered in terms of the blastomere polarity hypothesis and the involvement of the cytoskeleton with early embryonic development.

Original languageEnglish (US)
Pages (from-to)446-458
Number of pages13
JournalDevelopmental Biology
Volume115
Issue number2
DOIs
StatePublished - Jun 1986

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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