Abstract
We have examined cytoskeletal reorganization during early embryonic development in the hamster by employing detergent extraction to remove soluble components of the embryos and reveal the underlying structural network. This procedure allows examination of both the cortical cytoskeleton and the cytoskeleton of the egg interior. Sections of eggs and embryos were prepared for transmission electron microscopy with the removable embedding medium, diethylene glycol disterate which allows thicker sections than conventional embedment procedures thereby providing more spatial cues for studying organization. The cytoskeleton reorganizes after fertilization, at the time of compaction and again at the blastocyst stage. These cytoskeletal reorganizations are considered in terms of the blastomere polarity hypothesis and the involvement of the cytoskeleton with early embryonic development.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 446-458 |
| Number of pages | 13 |
| Journal | Developmental Biology |
| Volume | 115 |
| Issue number | 2 |
| DOIs | |
| State | Published - 1986 |
Fingerprint
ASJC Scopus subject areas
- Developmental Biology
Cite this
Cytoskeletal reorganization during early mammalian development : Analysis using embedment-free sections. / Capco, David; McGaughey, Robert W.
In: Developmental Biology, Vol. 115, No. 2, 1986, p. 446-458.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cytoskeletal reorganization during early mammalian development
T2 - Analysis using embedment-free sections
AU - Capco, David
AU - McGaughey, Robert W.
PY - 1986
Y1 - 1986
N2 - We have examined cytoskeletal reorganization during early embryonic development in the hamster by employing detergent extraction to remove soluble components of the embryos and reveal the underlying structural network. This procedure allows examination of both the cortical cytoskeleton and the cytoskeleton of the egg interior. Sections of eggs and embryos were prepared for transmission electron microscopy with the removable embedding medium, diethylene glycol disterate which allows thicker sections than conventional embedment procedures thereby providing more spatial cues for studying organization. The cytoskeleton reorganizes after fertilization, at the time of compaction and again at the blastocyst stage. These cytoskeletal reorganizations are considered in terms of the blastomere polarity hypothesis and the involvement of the cytoskeleton with early embryonic development.
AB - We have examined cytoskeletal reorganization during early embryonic development in the hamster by employing detergent extraction to remove soluble components of the embryos and reveal the underlying structural network. This procedure allows examination of both the cortical cytoskeleton and the cytoskeleton of the egg interior. Sections of eggs and embryos were prepared for transmission electron microscopy with the removable embedding medium, diethylene glycol disterate which allows thicker sections than conventional embedment procedures thereby providing more spatial cues for studying organization. The cytoskeleton reorganizes after fertilization, at the time of compaction and again at the blastocyst stage. These cytoskeletal reorganizations are considered in terms of the blastomere polarity hypothesis and the involvement of the cytoskeleton with early embryonic development.
UR - http://www.scopus.com/inward/record.url?scp=0022507295&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0022507295&partnerID=8YFLogxK
U2 - 10.1016/0012-1606(86)90265-4
DO - 10.1016/0012-1606(86)90265-4
M3 - Article
C2 - 3519319
AN - SCOPUS:0022507295
VL - 115
SP - 446
EP - 458
JO - Developmental Biology
JF - Developmental Biology
SN - 0012-1606
IS - 2
ER -