Cytological analysis of fertilization and early embryonic development in incompatible crosses of Drosophila simulans

Craig W. Lassy, Timothy L. Karr

Research output: Contribution to journalArticle

94 Citations (Scopus)

Abstract

Cytoplasmic incompatibility (CI) is a unique form of male sterility found in numerous insect species that harbor a bacterial endo-symbiont Wolbachia. CI is characterized by severe reduction in the progeny produced when infected males are crossed to uninfected females. The reduction in progeny correlates with developmental defects that arise during and immediately following fertilization, suggesting that sperm function is disrupted. To investigate the nature of the cellular defects associated with CI, fertilization and early embryonic development were examined in normal and incompatible crosses of Drosophila simulans using anti-sperm, anti-tubulin and anti-chromatin antibodies. Although pleiotropic, defects associated with CI can be classified into five broad categories: (1) sperm defects in the egg; (2) aberrant morphology of the mitotic apparatus; (3) defects in chromatin structure; (4) proliferation of centrosomes in the absence of nuclear division; and (5) loss of mitotic synchrony. Although mitosis and chromosome behavior are severely disrupted in CI crosses during early development, centrosome duplication and migration appear to continue unabated. The available cytological data suggest that the primary defects observed in incompatible crosses are due to defects in chromosome replication/segregation and in associated centrosome/microtubule-based processes.

Original languageEnglish (US)
Pages (from-to)47-58
Number of pages12
JournalMechanisms of Development
Volume57
Issue number1
DOIs
StatePublished - Jun 1996
Externally publishedYes

Fingerprint

Centrosome
Fertilization
Embryonic Development
Spermatozoa
Chromatin
Wolbachia
Cell Nucleus Division
Chromosome Segregation
Spindle Apparatus
Male Infertility
Tubulin
Mitosis
Microtubules
Ovum
Insects
Anti-Idiotypic Antibodies
Chromosomes
Drosophila simulans

Keywords

  • Cytoplasmic incompatibility
  • Egg lethality
  • Mitotic defect

ASJC Scopus subject areas

  • Embryology
  • Developmental Biology

Cite this

Cytological analysis of fertilization and early embryonic development in incompatible crosses of Drosophila simulans. / Lassy, Craig W.; Karr, Timothy L.

In: Mechanisms of Development, Vol. 57, No. 1, 06.1996, p. 47-58.

Research output: Contribution to journalArticle

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