Cytokinin from soluble RNA of Escherichia coli: 6-(3-methyl-2-butenylamino) -2-methylthio-9-β-D-ribofuranosylpurine

W. J. Burrows; D. J. Armstrong; F. Skoog; S. M. Hecht; J. T.A. Boyle; N. J. Leonard; J. Occolowitz

doi:10.1126/science.161.3842.691

Cytokinin from soluble RNA of Escherichia coli: 6-(3-methyl-2-butenylamino) -2-methylthio-9-β-D-ribofuranosylpurine

W. J. Burrows, D. J. Armstrong, F. Skoog, S. M. Hecht, J. T.A. Boyle, N. J. Leonard, J. Occolowitz

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Abstract

We have isolated a compound responsible for the cytokinin activity of soluble RNA from Escherichia coli. The structure, indicated as 6-(3-methyl-2-butenylamino)-2-methylthio-9-β-o-ribofuranosylpurine, C ₁₆H₂₃N₅O₄S, on the basis of low- and high-resolution mass spectrometry, was established by unequivocal synthesis. The mass spectra, chromatographic behavior, and ultraviolet spectra of the compounds from natural and synthetic sources were identical.

Original language	English (US)
Pages (from-to)	691-693
Number of pages	3
Journal	Science
Volume	161
Issue number	3842
DOIs	https://doi.org/10.1126/science.161.3842.691
State	Published - 1968
Externally published	Yes

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title = "Cytokinin from soluble RNA of Escherichia coli: 6-(3-methyl-2-butenylamino) -2-methylthio-9-β-D-ribofuranosylpurine",

abstract = "We have isolated a compound responsible for the cytokinin activity of soluble RNA from Escherichia coli. The structure, indicated as 6-(3-methyl-2-butenylamino)-2-methylthio-9-β-o-ribofuranosylpurine, C 16H23N5O4S, on the basis of low- and high-resolution mass spectrometry, was established by unequivocal synthesis. The mass spectra, chromatographic behavior, and ultraviolet spectra of the compounds from natural and synthetic sources were identical.",

author = "Burrows, {W. J.} and Armstrong, {D. J.} and F. Skoog and Hecht, {S. M.} and Boyle, {J. T.A.} and Leonard, {N. J.} and J. Occolowitz",

year = "1968",

doi = "10.1126/science.161.3842.691",

language = "English (US)",

volume = "161",

pages = "691--693",

journal = "Science",

issn = "0036-8075",

publisher = "American Association for the Advancement of Science",

number = "3842",

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TY - JOUR

T1 - Cytokinin from soluble RNA of Escherichia coli

T2 - 6-(3-methyl-2-butenylamino) -2-methylthio-9-β-D-ribofuranosylpurine

AU - Burrows, W. J.

AU - Armstrong, D. J.

AU - Skoog, F.

AU - Hecht, S. M.

AU - Boyle, J. T.A.

AU - Leonard, N. J.

AU - Occolowitz, J.

PY - 1968

Y1 - 1968

N2 - We have isolated a compound responsible for the cytokinin activity of soluble RNA from Escherichia coli. The structure, indicated as 6-(3-methyl-2-butenylamino)-2-methylthio-9-β-o-ribofuranosylpurine, C 16H23N5O4S, on the basis of low- and high-resolution mass spectrometry, was established by unequivocal synthesis. The mass spectra, chromatographic behavior, and ultraviolet spectra of the compounds from natural and synthetic sources were identical.

AB - We have isolated a compound responsible for the cytokinin activity of soluble RNA from Escherichia coli. The structure, indicated as 6-(3-methyl-2-butenylamino)-2-methylthio-9-β-o-ribofuranosylpurine, C 16H23N5O4S, on the basis of low- and high-resolution mass spectrometry, was established by unequivocal synthesis. The mass spectra, chromatographic behavior, and ultraviolet spectra of the compounds from natural and synthetic sources were identical.

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JF - Science

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ER -

Cytokinin from soluble RNA of Escherichia coli: 6-(3-methyl-2-butenylamino) -2-methylthio-9-β-D-ribofuranosylpurine

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