Cysteine-rich protein 1 (CRP1) regulates actin filament bundling

Thuan C. Tran, Corey Ayne Singleton, Tamara S. Fraley, Jeffrey A. Greenwood

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Background: Cysteine-rich protein 1 (CRP1) is a LIM domain containing protein localized to the nucleus and the actin cytoskeleton. CRP1 has been demonstrated to bind the actin-bundling protein α-actinin and proposed to modulate the actin cytoskeleton; however, specific regulatory mechanisms have not been identified. Results: CRP1 expression increased actin bundling in rat embryonic fibroblasts. Although CRP1 did not affect the bundling activity of α-actinin, CRP1 was found to stabilize the interaction of α-actinin with actin bundles and to directly bundle actin microfilaments. Using confocal and photobleaching fluorescence resonance energy transfer (FRET) microscopy, we demonstrate that there are two populations of CRP1 localized along actin stress fibers, one associated through interaction with α-actinin and one that appears to bind the actin filaments directly. Consistent with a role in regulating actin filament cross-linking, CRP1 also localized to the membrane ruffles of spreading and PDGF treated fibroblasts. Conclusions: CRP1 regulates actin filament bundling by directly cross-linking actin filaments and stabilizing the interaction of α-actinin with actin filament bundles.

Original languageEnglish (US)
Article number45
JournalBMC Cell Biology
Volume6
DOIs
StatePublished - Dec 8 2005
Externally publishedYes

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Actin Cytoskeleton
Cysteine
Actinin
Proteins
Actins
LIM Domain Proteins
Fibroblasts
Photobleaching
Stress Fibers
Fluorescence Resonance Energy Transfer
Microscopy
Membranes
Population

ASJC Scopus subject areas

  • Cell Biology

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Cysteine-rich protein 1 (CRP1) regulates actin filament bundling. / Tran, Thuan C.; Singleton, Corey Ayne; Fraley, Tamara S.; Greenwood, Jeffrey A.

In: BMC Cell Biology, Vol. 6, 45, 08.12.2005.

Research output: Contribution to journalArticle

Tran, Thuan C. ; Singleton, Corey Ayne ; Fraley, Tamara S. ; Greenwood, Jeffrey A. / Cysteine-rich protein 1 (CRP1) regulates actin filament bundling. In: BMC Cell Biology. 2005 ; Vol. 6.
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