Cysteine biosynthesis pathway in the archaeon Methanosarcina barkeri encoded by acquired bacterial genes?

Makoto Kitabatake, Man Wah So, Debra Hansen, Dieter Söll

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

The pathway of cysteine biosynthesis in archaea is still unexplored. Complementation of a cysteine auxotrophic Escherichia coli strain NK3 led to the isolation of the Methanosarcina barked cysK gene [encoding O-acetylserine (thiol)-lyase-A], which displays great similarity to bacterial cysK genes. Adjacent to cysK is an open reading frame orthologous to bacterial cysE (serine transacetylase) genes. These two genes could account for cysteine biosynthesis in this archaeon. Analysis of recent genome data revealed the presence of bacteria-like cysM genes [encoding O-acetylserine (thiol)-lyase- B] in Pyrococcus spp., Sulfolobus solfataricus, and Thermoplasma acidophilum. However, no orthologs for these genes can be found in Methanococcus jannaschii, Methanobacterium thermoautotrophicum, and Archaeoglobus fulgidus, implying the existence of unrecognizable genes for the same function or a different cysteine biosynthesis pathway.

Original languageEnglish (US)
Pages (from-to)143-145
Number of pages3
JournalJournal of Bacteriology
Volume182
Issue number1
DOIs
StatePublished - Jan 1 2000
Externally publishedYes

Fingerprint

Methanosarcina barkeri
Bacterial Genes
Archaea
Cysteine
Cysteine Synthase
Genes
Serine O-Acetyltransferase
Pyrococcus
Archaeoglobus fulgidus
Thermoplasma
Methanocaldococcus
Methanosarcina
Methanobacterium
Sulfolobus solfataricus
Open Reading Frames
Genome
Escherichia coli
Bacteria

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Cysteine biosynthesis pathway in the archaeon Methanosarcina barkeri encoded by acquired bacterial genes? / Kitabatake, Makoto; So, Man Wah; Hansen, Debra; Söll, Dieter.

In: Journal of Bacteriology, Vol. 182, No. 1, 01.01.2000, p. 143-145.

Research output: Contribution to journalArticle

Kitabatake, Makoto ; So, Man Wah ; Hansen, Debra ; Söll, Dieter. / Cysteine biosynthesis pathway in the archaeon Methanosarcina barkeri encoded by acquired bacterial genes?. In: Journal of Bacteriology. 2000 ; Vol. 182, No. 1. pp. 143-145.
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